Cell-specific expression of the IP3 receptor gene family in the kidney

Matsuhiko Hayashi, Toshiaki Monkawa, Takao Saruta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background/Aim: The localization of inositol trisphosphate (IP3) receptor isoforms, types 1-3, in the kidney and their role in the regulation of the intracellular calcium concentration - [Ca2+](i) - are discussed. Methods: Immunohistological studies with isoform-specific antibodies were performed to reveal the localization of IP3 receptor isoforms. To examine the role of IP3 receptor type 1 in the glomeruli, the responses of [Ca2+](i) to hormonal stimuli were examined in IP3 receptor type 1 knockout mice. Results: In the immunohistological study, type 1 receptor was present in arteries, afferent arterioles, and mesangial cells. Double staining with antibodies against aquaporin 2 and IP3 type 2 receptor revealed that type 2 receptor was localized mainly in the intercalated cells. The type 3 receptor showed characteristic intracellular localization in the collecting duct cells of the cortex to the outer medulla. Immunostaining of type 3 receptor was most intense in the cytoplasm on the basolateral membrane side and was not seen on the apical side. The responses of [Ca2+](i) to angiotensin II and endothelin in the glomeruli were markedly attenuated in IP3 receptor type 1 knockout mice. Conclusions: The three isoforms of the IP3 receptor showed distinctive localization in the kidney, and the type 1 receptor plays a major role in the regulation of [Ca2+](i) in the glomeruli. The physiological significance of the cell-specific localization, however, remains to be determined. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalExperimental Nephrology
Volume8
Issue number4-5
Publication statusPublished - 2000 Jul

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Kidney
Protein Isoforms
Genes
Knockout Mice
Aquaporin 2
Mesangial Cells
Antibodies
Endothelins
Arterioles
Inositol
Angiotensin II
Cytoplasm
Arteries
Staining and Labeling
Calcium
Membranes

Keywords

  • Immunohistochemistry
  • Inositol trisphosphate receptor
  • Isoforms
  • Kidney
  • Receptor localization

ASJC Scopus subject areas

  • Nephrology

Cite this

Cell-specific expression of the IP3 receptor gene family in the kidney. / Hayashi, Matsuhiko; Monkawa, Toshiaki; Saruta, Takao.

In: Experimental Nephrology, Vol. 8, No. 4-5, 07.2000, p. 215-218.

Research output: Contribution to journalArticle

Hayashi, Matsuhiko ; Monkawa, Toshiaki ; Saruta, Takao. / Cell-specific expression of the IP3 receptor gene family in the kidney. In: Experimental Nephrology. 2000 ; Vol. 8, No. 4-5. pp. 215-218.
@article{56fa4a29793749db805488db4de86e65,
title = "Cell-specific expression of the IP3 receptor gene family in the kidney",
abstract = "Background/Aim: The localization of inositol trisphosphate (IP3) receptor isoforms, types 1-3, in the kidney and their role in the regulation of the intracellular calcium concentration - [Ca2+](i) - are discussed. Methods: Immunohistological studies with isoform-specific antibodies were performed to reveal the localization of IP3 receptor isoforms. To examine the role of IP3 receptor type 1 in the glomeruli, the responses of [Ca2+](i) to hormonal stimuli were examined in IP3 receptor type 1 knockout mice. Results: In the immunohistological study, type 1 receptor was present in arteries, afferent arterioles, and mesangial cells. Double staining with antibodies against aquaporin 2 and IP3 type 2 receptor revealed that type 2 receptor was localized mainly in the intercalated cells. The type 3 receptor showed characteristic intracellular localization in the collecting duct cells of the cortex to the outer medulla. Immunostaining of type 3 receptor was most intense in the cytoplasm on the basolateral membrane side and was not seen on the apical side. The responses of [Ca2+](i) to angiotensin II and endothelin in the glomeruli were markedly attenuated in IP3 receptor type 1 knockout mice. Conclusions: The three isoforms of the IP3 receptor showed distinctive localization in the kidney, and the type 1 receptor plays a major role in the regulation of [Ca2+](i) in the glomeruli. The physiological significance of the cell-specific localization, however, remains to be determined. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "Immunohistochemistry, Inositol trisphosphate receptor, Isoforms, Kidney, Receptor localization",
author = "Matsuhiko Hayashi and Toshiaki Monkawa and Takao Saruta",
year = "2000",
month = "7",
language = "English",
volume = "8",
pages = "215--218",
journal = "Experimental Nephrology",
issn = "0028-2766",
publisher = "S. Karger AG",
number = "4-5",

}

TY - JOUR

T1 - Cell-specific expression of the IP3 receptor gene family in the kidney

AU - Hayashi, Matsuhiko

AU - Monkawa, Toshiaki

AU - Saruta, Takao

PY - 2000/7

Y1 - 2000/7

N2 - Background/Aim: The localization of inositol trisphosphate (IP3) receptor isoforms, types 1-3, in the kidney and their role in the regulation of the intracellular calcium concentration - [Ca2+](i) - are discussed. Methods: Immunohistological studies with isoform-specific antibodies were performed to reveal the localization of IP3 receptor isoforms. To examine the role of IP3 receptor type 1 in the glomeruli, the responses of [Ca2+](i) to hormonal stimuli were examined in IP3 receptor type 1 knockout mice. Results: In the immunohistological study, type 1 receptor was present in arteries, afferent arterioles, and mesangial cells. Double staining with antibodies against aquaporin 2 and IP3 type 2 receptor revealed that type 2 receptor was localized mainly in the intercalated cells. The type 3 receptor showed characteristic intracellular localization in the collecting duct cells of the cortex to the outer medulla. Immunostaining of type 3 receptor was most intense in the cytoplasm on the basolateral membrane side and was not seen on the apical side. The responses of [Ca2+](i) to angiotensin II and endothelin in the glomeruli were markedly attenuated in IP3 receptor type 1 knockout mice. Conclusions: The three isoforms of the IP3 receptor showed distinctive localization in the kidney, and the type 1 receptor plays a major role in the regulation of [Ca2+](i) in the glomeruli. The physiological significance of the cell-specific localization, however, remains to be determined. Copyright (C) 2000 S. Karger AG, Basel.

AB - Background/Aim: The localization of inositol trisphosphate (IP3) receptor isoforms, types 1-3, in the kidney and their role in the regulation of the intracellular calcium concentration - [Ca2+](i) - are discussed. Methods: Immunohistological studies with isoform-specific antibodies were performed to reveal the localization of IP3 receptor isoforms. To examine the role of IP3 receptor type 1 in the glomeruli, the responses of [Ca2+](i) to hormonal stimuli were examined in IP3 receptor type 1 knockout mice. Results: In the immunohistological study, type 1 receptor was present in arteries, afferent arterioles, and mesangial cells. Double staining with antibodies against aquaporin 2 and IP3 type 2 receptor revealed that type 2 receptor was localized mainly in the intercalated cells. The type 3 receptor showed characteristic intracellular localization in the collecting duct cells of the cortex to the outer medulla. Immunostaining of type 3 receptor was most intense in the cytoplasm on the basolateral membrane side and was not seen on the apical side. The responses of [Ca2+](i) to angiotensin II and endothelin in the glomeruli were markedly attenuated in IP3 receptor type 1 knockout mice. Conclusions: The three isoforms of the IP3 receptor showed distinctive localization in the kidney, and the type 1 receptor plays a major role in the regulation of [Ca2+](i) in the glomeruli. The physiological significance of the cell-specific localization, however, remains to be determined. Copyright (C) 2000 S. Karger AG, Basel.

KW - Immunohistochemistry

KW - Inositol trisphosphate receptor

KW - Isoforms

KW - Kidney

KW - Receptor localization

UR - http://www.scopus.com/inward/record.url?scp=0033839490&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033839490&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 215

EP - 218

JO - Experimental Nephrology

JF - Experimental Nephrology

SN - 0028-2766

IS - 4-5

ER -