Cell surface-associated aggregation-promoting factor from Lactobacillus gasseriSBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni

Keita Nishiyama, Akiko Nakazato, Shintaro Ueno, Yasuyuki Seto, Tsutomu Kakuda, Shinji Takai, Yuji Yamamoto, Takao Mukai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Campylobacter jejuni, one of the most common causes of gastroenteritis worldwide, is transmitted to humans through poultry. We previously reported that Lactobacillus gasseriSBT2055 (LG2055) reduced C. jejuni infection in human epithelial cells in vitro and inhibited pathogen colonization of chickens in vivo. This suggested that the LG2055 adhesion and/or co-aggregation phenotype mediated by cell-surface aggregation-promoting factors (APFs) may be important for the competitive exclusion of C. jejuni. Here, we show that cell surface-associated APF1 promoted LG2055 self-aggregation and adhesion to human epithelial cells and exhibited high affinity for the extracellular matrix component fibronectin. These effects were absent in the apf1 knockout mutant, indicating the role of APF1 in LG2055-mediated inhibition of C. jejuni in epithelial cells and chicken colonization. Similar to APF1, APF2 promoted the co-aggregation of LG2055 and C. jejuni but did not inhibit C. jejuni infection. Our data suggest a pivotal role for APF1 in mediating the interaction of LG2055 with human intestinal cells and in inhibiting C. jejuni colonization of the gastrointestinal tract. We thus provide new insight into the health-promoting effects of probiotics and mechanisms of competitive exclusion in poultry. Further research is needed to determine whether the probiotic strains reach the epithelial surface. We show that cell surface-associated aggregation-promoting factor (APF1) induced Lactobacillus gasseri SBT2055 (LG2055) self-aggregation and adhesion to human epithelial cells, and inhibited Campylobacter jejuni adhesion to and invasion of epithelial cells and chicken colonization. Our data suggest a pivotal role for APF1 in mediating LG2055 interaction with human intestinal cells and inhibiting C. jejuni colonization of the gastrointestinal tract, providing new insights into the health-promoting effects of probiotics.

Original languageEnglish
Pages (from-to)712-726
Number of pages15
JournalMolecular Microbiology
Volume98
Issue number4
DOIs
Publication statusPublished - 2015 Nov 1
Externally publishedYes

Fingerprint

Campylobacter jejuni
Lactobacillus
Epithelial Cells
Probiotics
Campylobacter Infections
Chickens
Poultry
Gastrointestinal Tract
cell aggregation factors
Cell Aggregation
Health
Gastroenteritis
Fibronectins
Extracellular Matrix
Phenotype

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cell surface-associated aggregation-promoting factor from Lactobacillus gasseriSBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni. / Nishiyama, Keita; Nakazato, Akiko; Ueno, Shintaro; Seto, Yasuyuki; Kakuda, Tsutomu; Takai, Shinji; Yamamoto, Yuji; Mukai, Takao.

In: Molecular Microbiology, Vol. 98, No. 4, 01.11.2015, p. 712-726.

Research output: Contribution to journalArticle

Nishiyama, Keita ; Nakazato, Akiko ; Ueno, Shintaro ; Seto, Yasuyuki ; Kakuda, Tsutomu ; Takai, Shinji ; Yamamoto, Yuji ; Mukai, Takao. / Cell surface-associated aggregation-promoting factor from Lactobacillus gasseriSBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni. In: Molecular Microbiology. 2015 ; Vol. 98, No. 4. pp. 712-726.
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AU - Seto, Yasuyuki

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AB - Campylobacter jejuni, one of the most common causes of gastroenteritis worldwide, is transmitted to humans through poultry. We previously reported that Lactobacillus gasseriSBT2055 (LG2055) reduced C. jejuni infection in human epithelial cells in vitro and inhibited pathogen colonization of chickens in vivo. This suggested that the LG2055 adhesion and/or co-aggregation phenotype mediated by cell-surface aggregation-promoting factors (APFs) may be important for the competitive exclusion of C. jejuni. Here, we show that cell surface-associated APF1 promoted LG2055 self-aggregation and adhesion to human epithelial cells and exhibited high affinity for the extracellular matrix component fibronectin. These effects were absent in the apf1 knockout mutant, indicating the role of APF1 in LG2055-mediated inhibition of C. jejuni in epithelial cells and chicken colonization. Similar to APF1, APF2 promoted the co-aggregation of LG2055 and C. jejuni but did not inhibit C. jejuni infection. Our data suggest a pivotal role for APF1 in mediating the interaction of LG2055 with human intestinal cells and in inhibiting C. jejuni colonization of the gastrointestinal tract. We thus provide new insight into the health-promoting effects of probiotics and mechanisms of competitive exclusion in poultry. Further research is needed to determine whether the probiotic strains reach the epithelial surface. We show that cell surface-associated aggregation-promoting factor (APF1) induced Lactobacillus gasseri SBT2055 (LG2055) self-aggregation and adhesion to human epithelial cells, and inhibited Campylobacter jejuni adhesion to and invasion of epithelial cells and chicken colonization. Our data suggest a pivotal role for APF1 in mediating LG2055 interaction with human intestinal cells and inhibiting C. jejuni colonization of the gastrointestinal tract, providing new insights into the health-promoting effects of probiotics.

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