Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes

Toshinori Sato, Tetsuya Ishizuka, Yoshio Okahata

Research output: Contribution to journalArticle

Abstract

We developed DNA complexes with glycolipids which have sugar and amine at both ends of alkyl chain. The molecular weights of the DNA/glycolipid complexes and the conformation of the DNA strands were observed by the multi-angle light scattering method. Sugar recognition properties of the DNA complexes were confirmed by the aggregation induced by the lectin such as ConA and RCA120. The DNA complex having a galactose terminal was specifically taken up by hepatoma HepG2 cell, furthermore, it showed increase in transfection efficiency. The cell uptakes were influenced by the variety of the sugars on the surface of the DNA complex. The transfection efficiencies were influenced by the stoichiometry of DNA and glycolipid in addition to the variety of sugars.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalKobunshi Ronbunshu
Volume55
Issue number4
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Glycolipids
DNA
Sugars
sugar
light scattering
stoichiometry
Galactose
Lectins
Stoichiometry
Light scattering
Amines
Conformations
Agglomeration
Molecular weight

Keywords

  • Carbohydrate Recognition
  • DNA Complex
  • Luciferase Assay
  • Transfection

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Environmental Science(all)
  • Materials Science (miscellaneous)
  • Ceramics and Composites
  • Polymers and Plastics

Cite this

Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes. / Sato, Toshinori; Ishizuka, Tetsuya; Okahata, Yoshio.

In: Kobunshi Ronbunshu, Vol. 55, No. 4, 1998, p. 217-224.

Research output: Contribution to journalArticle

Sato, T, Ishizuka, T & Okahata, Y 1998, 'Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes', Kobunshi Ronbunshu, vol. 55, no. 4, pp. 217-224.
Sato, Toshinori ; Ishizuka, Tetsuya ; Okahata, Yoshio. / Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes. In: Kobunshi Ronbunshu. 1998 ; Vol. 55, No. 4. pp. 217-224.
@article{7e6d04e8ee4e47389354d2a7dbe27e06,
title = "Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes",
abstract = "We developed DNA complexes with glycolipids which have sugar and amine at both ends of alkyl chain. The molecular weights of the DNA/glycolipid complexes and the conformation of the DNA strands were observed by the multi-angle light scattering method. Sugar recognition properties of the DNA complexes were confirmed by the aggregation induced by the lectin such as ConA and RCA120. The DNA complex having a galactose terminal was specifically taken up by hepatoma HepG2 cell, furthermore, it showed increase in transfection efficiency. The cell uptakes were influenced by the variety of the sugars on the surface of the DNA complex. The transfection efficiencies were influenced by the stoichiometry of DNA and glycolipid in addition to the variety of sugars.",
keywords = "Carbohydrate Recognition, DNA Complex, Luciferase Assay, Transfection",
author = "Toshinori Sato and Tetsuya Ishizuka and Yoshio Okahata",
year = "1998",
language = "English",
volume = "55",
pages = "217--224",
journal = "Kobunshi Ronbunshu",
issn = "0386-2186",
publisher = "Society of Polymer Science",
number = "4",

}

TY - JOUR

T1 - Cell Uptake and Transfection Efficiency of DNA/Glycolipid Complexes

AU - Sato, Toshinori

AU - Ishizuka, Tetsuya

AU - Okahata, Yoshio

PY - 1998

Y1 - 1998

N2 - We developed DNA complexes with glycolipids which have sugar and amine at both ends of alkyl chain. The molecular weights of the DNA/glycolipid complexes and the conformation of the DNA strands were observed by the multi-angle light scattering method. Sugar recognition properties of the DNA complexes were confirmed by the aggregation induced by the lectin such as ConA and RCA120. The DNA complex having a galactose terminal was specifically taken up by hepatoma HepG2 cell, furthermore, it showed increase in transfection efficiency. The cell uptakes were influenced by the variety of the sugars on the surface of the DNA complex. The transfection efficiencies were influenced by the stoichiometry of DNA and glycolipid in addition to the variety of sugars.

AB - We developed DNA complexes with glycolipids which have sugar and amine at both ends of alkyl chain. The molecular weights of the DNA/glycolipid complexes and the conformation of the DNA strands were observed by the multi-angle light scattering method. Sugar recognition properties of the DNA complexes were confirmed by the aggregation induced by the lectin such as ConA and RCA120. The DNA complex having a galactose terminal was specifically taken up by hepatoma HepG2 cell, furthermore, it showed increase in transfection efficiency. The cell uptakes were influenced by the variety of the sugars on the surface of the DNA complex. The transfection efficiencies were influenced by the stoichiometry of DNA and glycolipid in addition to the variety of sugars.

KW - Carbohydrate Recognition

KW - DNA Complex

KW - Luciferase Assay

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=0032050396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032050396&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032050396

VL - 55

SP - 217

EP - 224

JO - Kobunshi Ronbunshu

JF - Kobunshi Ronbunshu

SN - 0386-2186

IS - 4

ER -