Cellular differentiation of epithelioid sarcoma. An electron-microscopic, enzyme-histochemical, and immunohistochemical study

M. Mukai, C. Torikata, H. Iri, Hironari Hanaoka, T. Kawai, K. Yakumaru, T. Shimoda, A. Mikata, K. Kageyama

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Abstract

For the purpose of clarifying cellular differentiation of epithelioid sarcoma, studies based on various methods were performed. Enzyme histochemical studies showed that epithelioid sarcoma tumor cells have characteristics intermediate between epithelial cells and the large plump cells of synovial sarcoma - incomplete epithelial differentiation. For alkaline phosphatase and adenosine triphosphatase particularly, positive cells and negative cells coexisted, as in the large - plump cells of synovial sarcoma. Immunohistochemical studies for α1-antitrypsin, α1-antichymotrypsin, vimentin, and keratin also showed that epithelioid sarcoma tumor cells are very similar to the large plump cells of synovial sarcoma and have incomplete epithelial differentiation. For example, the examinations of serial sections and double staining methods revealed that keratin-positive cells are always vimentin-positive in epithelioid sarcoma and in the monophasic area of synovial sarcoma. Electron-microscopically, bundles of intermediate filaments and filopodia toward the intercellular lumen were observed, as in the monophasic area of synovial sarcoma. The results of enzyme-histochemical and immunohistochemical studies on non-neoplastic synovial lining cells, performed here for the first time, are also discussed.

Original languageEnglish
Pages (from-to)44-56
Number of pages13
JournalAmerican Journal of Pathology
Volume119
Issue number1
Publication statusPublished - 1985 Jul 31
Externally publishedYes

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Sarcoma
Synovial Sarcoma
Electrons
Enzymes
Vimentin
Keratins
Pseudopodia
Intermediate Filaments
Alkaline Phosphatase
Adenosine Triphosphatases
Neoplasms
Epithelial Cells
Staining and Labeling

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cellular differentiation of epithelioid sarcoma. An electron-microscopic, enzyme-histochemical, and immunohistochemical study. / Mukai, M.; Torikata, C.; Iri, H.; Hanaoka, Hironari; Kawai, T.; Yakumaru, K.; Shimoda, T.; Mikata, A.; Kageyama, K.

In: American Journal of Pathology, Vol. 119, No. 1, 31.07.1985, p. 44-56.

Research output: Contribution to journalArticle

Mukai, M, Torikata, C, Iri, H, Hanaoka, H, Kawai, T, Yakumaru, K, Shimoda, T, Mikata, A & Kageyama, K 1985, 'Cellular differentiation of epithelioid sarcoma. An electron-microscopic, enzyme-histochemical, and immunohistochemical study', American Journal of Pathology, vol. 119, no. 1, pp. 44-56.
Mukai, M. ; Torikata, C. ; Iri, H. ; Hanaoka, Hironari ; Kawai, T. ; Yakumaru, K. ; Shimoda, T. ; Mikata, A. ; Kageyama, K. / Cellular differentiation of epithelioid sarcoma. An electron-microscopic, enzyme-histochemical, and immunohistochemical study. In: American Journal of Pathology. 1985 ; Vol. 119, No. 1. pp. 44-56.
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