Cellular dynamics of bovine aortic smooth muscle cells measured using MEMS force sensors

Takuya Tsukagoshi, Thanh Vinh Nguyen, Kayoko Hirayama Shoji, Hidetoshi Takahashi, Kiyoshi Matsumoto, Isao Shimoyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Adhesive cells perceive the mechanical properties of the substrates to which they adhere, adjusting their cellular mechanical forces according to their biological characteristics. This mechanical interaction subsequently affects the growth, locomotion, and differentiation of the cell. However, little is known about the detailed mechanism that underlies this interaction between adherent cells and substrates because dynamically measuring mechanical phenomena is difficult. Here, we utilize microelectromechamical systems force sensors that can measure cellular traction forces with high temporal resolution (∼2.5 μs) over long periods (∼3 h). We found that the cellular dynamics reflected physical phenomena with time scales from milliseconds to hours, which contradicts the idea that cellular motion is slow. A single focal adhesion (FA) generates an average force of 7 nN, which disappears in ms via the action of trypsin-ethylenediaminetetraacetic acid. The force-changing rate obtained from our measurements suggests that the time required for an FA to decompose was nearly proportional to the force acting on the FA.

Original languageEnglish
Article number145401
JournalJournal of Physics D: Applied Physics
Volume51
Issue number14
DOIs
Publication statusPublished - 2018 Mar 12
Externally publishedYes

Fingerprint

smooth muscle
muscle cells
microelectromechanical systems
MEMS
Muscle
Adhesion
Cells
sensors
Sensors
adhesion
Ethylenediaminetetraacetic acid
Substrates
Edetic Acid
Trypsin
Adhesives
cells
trypsin
Mechanical properties
locomotion
ethylenediaminetetraacetic acids

Keywords

  • cellular dynamics
  • focal adhesion
  • piezoresistive cantilever

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this

Cellular dynamics of bovine aortic smooth muscle cells measured using MEMS force sensors. / Tsukagoshi, Takuya; Nguyen, Thanh Vinh; Shoji, Kayoko Hirayama; Takahashi, Hidetoshi; Matsumoto, Kiyoshi; Shimoyama, Isao.

In: Journal of Physics D: Applied Physics, Vol. 51, No. 14, 145401, 12.03.2018.

Research output: Contribution to journalArticle

Tsukagoshi, Takuya ; Nguyen, Thanh Vinh ; Shoji, Kayoko Hirayama ; Takahashi, Hidetoshi ; Matsumoto, Kiyoshi ; Shimoyama, Isao. / Cellular dynamics of bovine aortic smooth muscle cells measured using MEMS force sensors. In: Journal of Physics D: Applied Physics. 2018 ; Vol. 51, No. 14.
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