Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons

Cristina Bäckman, Barry J. Hoffer, Hidemi Misawa, Marisela Morales

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previous studies have shown that NGFI-B mRNA is highly expressed in the adult striatum. In the present study we analyzed the anatomical distribution of NGFI-B mRNA within this brain region as well as the degree of co-existence of NGFI-B with different striatal markers in the adult brain. NGFI-B mRNA levels were found to be significantly higher within the dorsomedial portion of the striatum as compared to the ventrolateral striatum. This distribution pattern was maintained throughout the rostro-caudal axis of the striatum. Double in situ hybridization studies showed that striatal NGFI-B mRNA colocalized with a subset of preproenkephalin and prodynorphin positive spiny neurons within the dorsomedial striatum; 22-28% of all opiate-peptide positive cells co-expressed NGFI-B mRNA. NGFI-B did not colocalize with striatal aspiny interneurons expressing choline acetyl transferase mRNA or those containing the calcium-binding protein parvalbumin. The pattern of NGFI-B mRNA expression within different striatal spiny projecting neurons suggests that this transcription factor may have a direct effect on the function of different striatal efferent pathways.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalBrain Research
Volume903
Issue number1-2
DOIs
Publication statusPublished - 2001 Jun 8
Externally publishedYes

Fingerprint

Corpus Striatum
Opioid Peptides
Regulator Genes
Transcription Factors
Neurons
Messenger RNA
Efferent Pathways
Parvalbumins
Calcium-Binding Proteins
Brain
Interneurons
Transferases
Choline
In Situ Hybridization

Keywords

  • Distribution
  • Dynorphin
  • Enkephalin
  • NGFI-B
  • Nur77
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons. / Bäckman, Cristina; Hoffer, Barry J.; Misawa, Hidemi; Morales, Marisela.

In: Brain Research, Vol. 903, No. 1-2, 08.06.2001, p. 26-32.

Research output: Contribution to journalArticle

Bäckman, Cristina ; Hoffer, Barry J. ; Misawa, Hidemi ; Morales, Marisela. / Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons. In: Brain Research. 2001 ; Vol. 903, No. 1-2. pp. 26-32.
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