Abstract
Previous studies have shown that NGFI-B mRNA is highly expressed in the adult striatum. In the present study we analyzed the anatomical distribution of NGFI-B mRNA within this brain region as well as the degree of co-existence of NGFI-B with different striatal markers in the adult brain. NGFI-B mRNA levels were found to be significantly higher within the dorsomedial portion of the striatum as compared to the ventrolateral striatum. This distribution pattern was maintained throughout the rostro-caudal axis of the striatum. Double in situ hybridization studies showed that striatal NGFI-B mRNA colocalized with a subset of preproenkephalin and prodynorphin positive spiny neurons within the dorsomedial striatum; 22-28% of all opiate-peptide positive cells co-expressed NGFI-B mRNA. NGFI-B did not colocalize with striatal aspiny interneurons expressing choline acetyl transferase mRNA or those containing the calcium-binding protein parvalbumin. The pattern of NGFI-B mRNA expression within different striatal spiny projecting neurons suggests that this transcription factor may have a direct effect on the function of different striatal efferent pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 26-32 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 903 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2001 Jun 8 |
| Externally published | Yes |
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Keywords
- Distribution
- Dynorphin
- Enkephalin
- NGFI-B
- Nur77
- Striatum
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons. / Bäckman, Cristina; Hoffer, Barry J.; Misawa, Hidemi; Morales, Marisela.
In: Brain Research, Vol. 903, No. 1-2, 08.06.2001, p. 26-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons
AU - Bäckman, Cristina
AU - Hoffer, Barry J.
AU - Misawa, Hidemi
AU - Morales, Marisela
PY - 2001/6/8
Y1 - 2001/6/8
N2 - Previous studies have shown that NGFI-B mRNA is highly expressed in the adult striatum. In the present study we analyzed the anatomical distribution of NGFI-B mRNA within this brain region as well as the degree of co-existence of NGFI-B with different striatal markers in the adult brain. NGFI-B mRNA levels were found to be significantly higher within the dorsomedial portion of the striatum as compared to the ventrolateral striatum. This distribution pattern was maintained throughout the rostro-caudal axis of the striatum. Double in situ hybridization studies showed that striatal NGFI-B mRNA colocalized with a subset of preproenkephalin and prodynorphin positive spiny neurons within the dorsomedial striatum; 22-28% of all opiate-peptide positive cells co-expressed NGFI-B mRNA. NGFI-B did not colocalize with striatal aspiny interneurons expressing choline acetyl transferase mRNA or those containing the calcium-binding protein parvalbumin. The pattern of NGFI-B mRNA expression within different striatal spiny projecting neurons suggests that this transcription factor may have a direct effect on the function of different striatal efferent pathways.
AB - Previous studies have shown that NGFI-B mRNA is highly expressed in the adult striatum. In the present study we analyzed the anatomical distribution of NGFI-B mRNA within this brain region as well as the degree of co-existence of NGFI-B with different striatal markers in the adult brain. NGFI-B mRNA levels were found to be significantly higher within the dorsomedial portion of the striatum as compared to the ventrolateral striatum. This distribution pattern was maintained throughout the rostro-caudal axis of the striatum. Double in situ hybridization studies showed that striatal NGFI-B mRNA colocalized with a subset of preproenkephalin and prodynorphin positive spiny neurons within the dorsomedial striatum; 22-28% of all opiate-peptide positive cells co-expressed NGFI-B mRNA. NGFI-B did not colocalize with striatal aspiny interneurons expressing choline acetyl transferase mRNA or those containing the calcium-binding protein parvalbumin. The pattern of NGFI-B mRNA expression within different striatal spiny projecting neurons suggests that this transcription factor may have a direct effect on the function of different striatal efferent pathways.
KW - Distribution
KW - Dynorphin
KW - Enkephalin
KW - NGFI-B
KW - Nur77
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=0035827080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035827080&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(01)02332-0
DO - 10.1016/S0006-8993(01)02332-0
M3 - Article
VL - 903
SP - 26
EP - 32
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -