Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature

Ryu Yamanaka, Yutaka Shindo, Kohji Hotta, Noriko Hiroi, Kotaro Oka

Research output: Contribution to journalArticle

Abstract

Temperature governs states and dynamics of all biological molecules, and several cellular processes are often heat sources and/or sinks. Technical achievement of intracellular thermometry enables us to measure intracellular temperature, and it can offer novel perspectives in biology and medicine. However, little is known that changes of intracellular temperature throughout the cell-cycle and the manner of which cells regulates their thermogenesis in response to fluctuation of the environmental temperature. Here, cell-cycle-dependent changes of intracellular temperature were reconstructed from the snapshots of cell population at single-cell resolution using ergodic analysis for asynchronously cultured HeLa cells expressing a genetically encoded thermometry. Intracellular temperature is highest at G1 phase, and it gradually decreases along cell-cycle progression and increases abruptly during mitosis. Cells easily heated up are harder to cool down and vice versa, especially at G1/S phases. Together, intracellular thermogenesis depends on cell-cycle phases and it maintains intracellular temperature through compensating environmental temperature fluctuations.

Original languageEnglish
Pages (from-to)70-76
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume533
Issue number1
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Cell cycle
  • Fluorescence imaging
  • Intracellular temperature
  • Thermogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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