Centrifugal microfluidic system for multistep assay using small amount of various samples

H. Hasegawa; T. Teshima; H. Onoe; S. Takeuchi

doi:10.1016/j.snb.2014.01.024

Centrifugal microfluidic system for multistep assay using small amount of various samples

H. Hasegawa, T. Teshima, H. Onoe, S. Takeuchi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

This paper describes a centrifuge-based dynamic microarray system using small amount of samples for application in multistep assays. The system uses meander-shaped microchannel and arrayed trapping spots to trap the samples with fluid stream generated by centrifugal force. Producing an array of samples suspended in only 1 μl of solution is achieved in 3 s with this system. After the loading solution is introduced, air is infused into the microchannel, keeping the sample and loading solution at the trapping spot. This air infusion allows us to immobilize and isolate the trapped samples suspended in the loading solution. In addition, the solution around the trapped sample can be repeatedly exchanged by introducing another solution. This system is applicable for multistep assays using small amount of rare samples.

Original language	English
Pages (from-to)	281-286
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	195
DOIs	https://doi.org/10.1016/j.snb.2014.01.024
Publication status	Published - 2014 May
Externally published	Yes

Keywords

Bead-based assay
Centrifugal force
Dynamic microarray
Multistep assay

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2014.01.024

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title = "Centrifugal microfluidic system for multistep assay using small amount of various samples",

abstract = "This paper describes a centrifuge-based dynamic microarray system using small amount of samples for application in multistep assays. The system uses meander-shaped microchannel and arrayed trapping spots to trap the samples with fluid stream generated by centrifugal force. Producing an array of samples suspended in only 1 μl of solution is achieved in 3 s with this system. After the loading solution is introduced, air is infused into the microchannel, keeping the sample and loading solution at the trapping spot. This air infusion allows us to immobilize and isolate the trapped samples suspended in the loading solution. In addition, the solution around the trapped sample can be repeatedly exchanged by introducing another solution. This system is applicable for multistep assays using small amount of rare samples.",

keywords = "Bead-based assay, Centrifugal force, Dynamic microarray, Multistep assay",

author = "H. Hasegawa and T. Teshima and H. Onoe and S. Takeuchi",

note = "Funding Information: Shoji Takeuchi received his Dr. Eng. degree in mechanical engineering in 2000 from the University of Tokyo. He is currently an associate professor in the Center for International Research on Micronano Mechatronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo. He has received several awards including the Advanced Research from the Japan Society of Medical Electronics and Biological Engineering in 2001, Young Scientists{\textquoteright} Prize, the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology in 2008, and the JSPS prize from the Japan Society for the Promotion of Science in 2010. Funding Information: Tetsuhiko Teshima received his B.S. degree in Faculty of Agriculture, and MS degree in School of Arts and Science from The University of Tokyo, Japan in 2009 and 2011, respectively. He had received Japan Society Promotion and Science (JSPS) Research Fellowship from 2011 to 2014. He is currently working toward the Ph.D. degree in the Department of Mechano-Informatics, School of Information Science and Technology at The University of Tokyo. ",

year = "2014",

month = may,

doi = "10.1016/j.snb.2014.01.024",

language = "English",

volume = "195",

pages = "281--286",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

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AU - Hasegawa, H.

AU - Teshima, T.

AU - Onoe, H.

AU - Takeuchi, S.

N1 - Funding Information: Shoji Takeuchi received his Dr. Eng. degree in mechanical engineering in 2000 from the University of Tokyo. He is currently an associate professor in the Center for International Research on Micronano Mechatronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo. He has received several awards including the Advanced Research from the Japan Society of Medical Electronics and Biological Engineering in 2001, Young Scientists’ Prize, the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology in 2008, and the JSPS prize from the Japan Society for the Promotion of Science in 2010. Funding Information: Tetsuhiko Teshima received his B.S. degree in Faculty of Agriculture, and MS degree in School of Arts and Science from The University of Tokyo, Japan in 2009 and 2011, respectively. He had received Japan Society Promotion and Science (JSPS) Research Fellowship from 2011 to 2014. He is currently working toward the Ph.D. degree in the Department of Mechano-Informatics, School of Information Science and Technology at The University of Tokyo.

PY - 2014/5

Y1 - 2014/5

N2 - This paper describes a centrifuge-based dynamic microarray system using small amount of samples for application in multistep assays. The system uses meander-shaped microchannel and arrayed trapping spots to trap the samples with fluid stream generated by centrifugal force. Producing an array of samples suspended in only 1 μl of solution is achieved in 3 s with this system. After the loading solution is introduced, air is infused into the microchannel, keeping the sample and loading solution at the trapping spot. This air infusion allows us to immobilize and isolate the trapped samples suspended in the loading solution. In addition, the solution around the trapped sample can be repeatedly exchanged by introducing another solution. This system is applicable for multistep assays using small amount of rare samples.

AB - This paper describes a centrifuge-based dynamic microarray system using small amount of samples for application in multistep assays. The system uses meander-shaped microchannel and arrayed trapping spots to trap the samples with fluid stream generated by centrifugal force. Producing an array of samples suspended in only 1 μl of solution is achieved in 3 s with this system. After the loading solution is introduced, air is infused into the microchannel, keeping the sample and loading solution at the trapping spot. This air infusion allows us to immobilize and isolate the trapped samples suspended in the loading solution. In addition, the solution around the trapped sample can be repeatedly exchanged by introducing another solution. This system is applicable for multistep assays using small amount of rare samples.

KW - Bead-based assay

KW - Centrifugal force

KW - Dynamic microarray

KW - Multistep assay

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JO - Sensors and Actuators B: Chemical

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Centrifugal microfluidic system for multistep assay using small amount of various samples

Abstract

Keywords

ASJC Scopus subject areas

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