Centrifuge-based cell encapsulation in hydrogel microbeads using sub-microliter sample solution

Hiroaki Onoe, Kiichi Inamori, Masahiro Takinoue, Shoji Takeuchi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This paper describes a simple, rapid, and inexpensive method for cell encapsulation that uses a small amount (sub-microliter-scale) of cell-suspended pre-gel solution. This method requires only a glass capillary, an acrylic holder, and a tabletop centrifuge, and achieves cell encapsulation in calcium alginate microbeads using cell-suspended sub-microliter-volume pre-gel solution in 20 s. The method also ensures high cell encapsulation efficiency, with a sample loss of as low as several tens of nanoliters. Formation of calcium alginate hydrogel microbeads encapsulating Jurkat cells by the proposed method is experimentally demonstrated using 0.5 μL of cell-suspended sodium alginate solution. Our proposed method is expected to be a promising approach for encapsulating rare and precious samples by non-specialists of microfluidics working in the fields of point-of-care and tailor-made medicine.

Original languageEnglish
Pages (from-to)30480-30484
Number of pages5
JournalRSC Advances
Volume4
Issue number58
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Hydrogel
Centrifuges
Encapsulation
Hydrogels
Alginate
Calcium
Gels
Sodium alginate
Microfluidics
Acrylics
Medicine
Glass
alginic acid

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Centrifuge-based cell encapsulation in hydrogel microbeads using sub-microliter sample solution. / Onoe, Hiroaki; Inamori, Kiichi; Takinoue, Masahiro; Takeuchi, Shoji.

In: RSC Advances, Vol. 4, No. 58, 2014, p. 30480-30484.

Research output: Contribution to journalArticle

Onoe, Hiroaki ; Inamori, Kiichi ; Takinoue, Masahiro ; Takeuchi, Shoji. / Centrifuge-based cell encapsulation in hydrogel microbeads using sub-microliter sample solution. In: RSC Advances. 2014 ; Vol. 4, No. 58. pp. 30480-30484.
@article{381583138a35497380988502156596c2,
title = "Centrifuge-based cell encapsulation in hydrogel microbeads using sub-microliter sample solution",
abstract = "This paper describes a simple, rapid, and inexpensive method for cell encapsulation that uses a small amount (sub-microliter-scale) of cell-suspended pre-gel solution. This method requires only a glass capillary, an acrylic holder, and a tabletop centrifuge, and achieves cell encapsulation in calcium alginate microbeads using cell-suspended sub-microliter-volume pre-gel solution in 20 s. The method also ensures high cell encapsulation efficiency, with a sample loss of as low as several tens of nanoliters. Formation of calcium alginate hydrogel microbeads encapsulating Jurkat cells by the proposed method is experimentally demonstrated using 0.5 μL of cell-suspended sodium alginate solution. Our proposed method is expected to be a promising approach for encapsulating rare and precious samples by non-specialists of microfluidics working in the fields of point-of-care and tailor-made medicine.",
author = "Hiroaki Onoe and Kiichi Inamori and Masahiro Takinoue and Shoji Takeuchi",
year = "2014",
doi = "10.1039/c4ra02773f",
language = "English",
volume = "4",
pages = "30480--30484",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "58",

}

TY - JOUR

T1 - Centrifuge-based cell encapsulation in hydrogel microbeads using sub-microliter sample solution

AU - Onoe, Hiroaki

AU - Inamori, Kiichi

AU - Takinoue, Masahiro

AU - Takeuchi, Shoji

PY - 2014

Y1 - 2014

N2 - This paper describes a simple, rapid, and inexpensive method for cell encapsulation that uses a small amount (sub-microliter-scale) of cell-suspended pre-gel solution. This method requires only a glass capillary, an acrylic holder, and a tabletop centrifuge, and achieves cell encapsulation in calcium alginate microbeads using cell-suspended sub-microliter-volume pre-gel solution in 20 s. The method also ensures high cell encapsulation efficiency, with a sample loss of as low as several tens of nanoliters. Formation of calcium alginate hydrogel microbeads encapsulating Jurkat cells by the proposed method is experimentally demonstrated using 0.5 μL of cell-suspended sodium alginate solution. Our proposed method is expected to be a promising approach for encapsulating rare and precious samples by non-specialists of microfluidics working in the fields of point-of-care and tailor-made medicine.

AB - This paper describes a simple, rapid, and inexpensive method for cell encapsulation that uses a small amount (sub-microliter-scale) of cell-suspended pre-gel solution. This method requires only a glass capillary, an acrylic holder, and a tabletop centrifuge, and achieves cell encapsulation in calcium alginate microbeads using cell-suspended sub-microliter-volume pre-gel solution in 20 s. The method also ensures high cell encapsulation efficiency, with a sample loss of as low as several tens of nanoliters. Formation of calcium alginate hydrogel microbeads encapsulating Jurkat cells by the proposed method is experimentally demonstrated using 0.5 μL of cell-suspended sodium alginate solution. Our proposed method is expected to be a promising approach for encapsulating rare and precious samples by non-specialists of microfluidics working in the fields of point-of-care and tailor-made medicine.

UR - http://www.scopus.com/inward/record.url?scp=84904788601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904788601&partnerID=8YFLogxK

U2 - 10.1039/c4ra02773f

DO - 10.1039/c4ra02773f

M3 - Article

AN - SCOPUS:84904788601

VL - 4

SP - 30480

EP - 30484

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 58

ER -