Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples

Kiichi Inamori, Hiroaki Onoe, Masahiro Takinoue, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a simple, rapid and inexpensive cell encapsulation method using ultra low volume (submicroliter) of cell-suspended pre-gel solution. This method requires only a glass capillary, acrylic holder and tabletop centrifuge, and 20 seconds to complete single cell encapsulation that ensures high viability of encapsulated cells and high efficiency of cell encapsulation. Our cell encapsulation method would be a promising tool for encapsulating rare and precious samples in biological analyses, and would conveniently be acceptable to non-specialists of microfluidics working in biological research and point-of-care medicine.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages314-316
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Hydrogel
Centrifuges
Encapsulation
Hydrogels
Microfluidics
Acrylics
Medicine
Gels
Glass

Keywords

  • Calcium alginate
  • Centrifuge
  • Encapsulation
  • Hydrogel
  • Single cell

ASJC Scopus subject areas

  • Bioengineering

Cite this

Inamori, K., Onoe, H., Takinoue, M., & Takeuchi, S. (2013). Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 314-316). Chemical and Biological Microsystems Society.

Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. / Inamori, Kiichi; Onoe, Hiroaki; Takinoue, Masahiro; Takeuchi, Shoji.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 314-316.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Inamori, K, Onoe, H, Takinoue, M & Takeuchi, S 2013, Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 314-316, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Inamori K, Onoe H, Takinoue M, Takeuchi S. Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 314-316
Inamori, Kiichi ; Onoe, Hiroaki ; Takinoue, Masahiro ; Takeuchi, Shoji. / Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 314-316
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