Centrosome amplification in adult T-cell leukemia and human T-cell leukemia virus type 1 Tax-induced human T cells

Takayuki Nitta, Masayuki Kanai, Eiji Sugihara, Masakazu Tanaka, Binilian Sun, Toshiro Nagasawa, Shunro Sonoda, Hideyuki Saya, Masanao Miwa

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Centrosomes play pivotal roles in cell polarity, regulation of the cell cycle and chromosomal segregation. Centrosome amplification was recently described as a possible cause of aneuploidy in certain solid tumors and leukemias. ATL is a T-cell malignancy caused by HTLV-1. Although the precise mechanism of cell transformation is unclear, the HTLV-1-encoded protein, Tax, is thought to play a crucial role in leukemogenesis. Here we demonstrate that lymphocytes isolated from patients with ATL show centrosome amplification and that a human T cell line shows centrosome amplification after induction of Tax, which was suppressed by CDK inhibitors. Micronuclei formation was also observed after centrosome amplification in Tax-induced human T cells. These findings suggest that Tax deregulates CDK activity and induces centrosome amplification, which might be associated with cellular transformation by HTLV-1 and chromosomal instability in HTLV-1-infected human T cells.

Original languageEnglish
Pages (from-to)836-841
Number of pages6
JournalCancer Science
Volume97
Issue number9
DOIs
Publication statusPublished - 2006 Sep
Externally publishedYes

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Deltaretrovirus
Adult T Cell Leukemia Lymphoma
Centrosome
Human T-lymphotropic virus 1
T-Lymphocytes
tax Gene Products
Cell Polarity
Chromosomal Instability
Aneuploidy
Neoplasms
Cell Cycle
Leukemia
Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Centrosome amplification in adult T-cell leukemia and human T-cell leukemia virus type 1 Tax-induced human T cells. / Nitta, Takayuki; Kanai, Masayuki; Sugihara, Eiji; Tanaka, Masakazu; Sun, Binilian; Nagasawa, Toshiro; Sonoda, Shunro; Saya, Hideyuki; Miwa, Masanao.

In: Cancer Science, Vol. 97, No. 9, 09.2006, p. 836-841.

Research output: Contribution to journalArticle

Nitta, T, Kanai, M, Sugihara, E, Tanaka, M, Sun, B, Nagasawa, T, Sonoda, S, Saya, H & Miwa, M 2006, 'Centrosome amplification in adult T-cell leukemia and human T-cell leukemia virus type 1 Tax-induced human T cells', Cancer Science, vol. 97, no. 9, pp. 836-841. https://doi.org/10.1111/j.1349-7006.2006.00254.x
Nitta, Takayuki ; Kanai, Masayuki ; Sugihara, Eiji ; Tanaka, Masakazu ; Sun, Binilian ; Nagasawa, Toshiro ; Sonoda, Shunro ; Saya, Hideyuki ; Miwa, Masanao. / Centrosome amplification in adult T-cell leukemia and human T-cell leukemia virus type 1 Tax-induced human T cells. In: Cancer Science. 2006 ; Vol. 97, No. 9. pp. 836-841.
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