Abstract
As a first step toward identifying the factor(s) that is/are produced by astrocytes and support(s) the survival of cerebellar Purkinje cells in dissociated culture, we compared the effect of astrocytes of cerebellar, hippocampal, and cerebral origin. A feeder coverslip of cerebellar astrocytes, which did not have cell-to-cell contact to neuronal culture, increased the percentage of Purkinje cells to about 8-9 fold with no change in the percentage of astrocytes. On the other hand, astrocytes of hippocampal or cerebral origin did not increase the percentage of Purkinje cells at low plating density, whereas they increased the number of astrocytes in neuronal culture. These results indicate that the factor(s), tentatively named as Purkinje-cell suvival factor was/were specifically produced by cerebellar astrocytes and affected directly on Purkinje cells.
| Original language | English |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 197 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1993 Nov 30 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Cerebellar Astrocytes Specifically Support the Survival of Purkinje Cells in Culture. / Yuzaki, Michisuke; Mikoshiba, K.; Kagawa, Y.
In: Biochemical and Biophysical Research Communications, Vol. 197, No. 1, 30.11.1993, p. 123-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cerebellar Astrocytes Specifically Support the Survival of Purkinje Cells in Culture
AU - Yuzaki, Michisuke
AU - Mikoshiba, K.
AU - Kagawa, Y.
PY - 1993/11/30
Y1 - 1993/11/30
N2 - As a first step toward identifying the factor(s) that is/are produced by astrocytes and support(s) the survival of cerebellar Purkinje cells in dissociated culture, we compared the effect of astrocytes of cerebellar, hippocampal, and cerebral origin. A feeder coverslip of cerebellar astrocytes, which did not have cell-to-cell contact to neuronal culture, increased the percentage of Purkinje cells to about 8-9 fold with no change in the percentage of astrocytes. On the other hand, astrocytes of hippocampal or cerebral origin did not increase the percentage of Purkinje cells at low plating density, whereas they increased the number of astrocytes in neuronal culture. These results indicate that the factor(s), tentatively named as Purkinje-cell suvival factor was/were specifically produced by cerebellar astrocytes and affected directly on Purkinje cells.
AB - As a first step toward identifying the factor(s) that is/are produced by astrocytes and support(s) the survival of cerebellar Purkinje cells in dissociated culture, we compared the effect of astrocytes of cerebellar, hippocampal, and cerebral origin. A feeder coverslip of cerebellar astrocytes, which did not have cell-to-cell contact to neuronal culture, increased the percentage of Purkinje cells to about 8-9 fold with no change in the percentage of astrocytes. On the other hand, astrocytes of hippocampal or cerebral origin did not increase the percentage of Purkinje cells at low plating density, whereas they increased the number of astrocytes in neuronal culture. These results indicate that the factor(s), tentatively named as Purkinje-cell suvival factor was/were specifically produced by cerebellar astrocytes and affected directly on Purkinje cells.
UR - http://www.scopus.com/inward/record.url?scp=0027374644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027374644&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2450
DO - 10.1006/bbrc.1993.2450
M3 - Article
C2 - 8250916
AN - SCOPUS:0027374644
VL - 197
SP - 123
EP - 129
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -