Cerebellar astrocytes specifically support the survival of purkinje cells in culture

Michisuke Yuzaki, Katsuhiko Mikoshiba, Yasuo Kagawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As a first step toward identifying the factor(s) that is/are produced by astrocytes and support(s) the survival of cerebellar Purkinje cells in dissociated culture, we compared the effect of astrocytes of cerebellar, hippocampal, and cerebral origin. A feeder coverslip of cerebellar astrocytes, which did not have cell-to-cell contact to neuronal culture, increased the percentage of Purkinje cells to about 8-9 fold with no change in the percentage of astrocytes. On the other hand, astrocytes of hippocampal or cerebral origin did not increase the percentage of Purkinje cells at low plating density, whereas they increased the number of astrocytes in neuronal culture. These results indicate that the factor(s), tentatively named as Purkinje-cell suvival factor was/were specifically produced by cerebellar astrocytes and affected directly on Purkinje cells.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume197
Issue number1
DOIs
Publication statusPublished - 1993 Nov 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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