Cerebellar long-term depression requires dephosphorylation of TARP in Purkinje cells

Toshihiro Nomura, Wataru Kakegawa, Shinji Matsuda, Kazuhisa Kohda, Jun Nishiyama, Takao Takahashi, Michisuke Yuzaki

Research output: Contribution to journalArticle

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Abstract

Cerebellar long-term depression (LTD) at parallel fiber (PF)-Purkinje cell synapses is thought to play an essential role in certain forms of motor learning. Like hippocampal LTD, cerebellar LTD is mediated by the endocytosis of AMPA (α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate) receptors at postsynaptic sites. However, similar sets of kinases and phosphatases have opposite regulatory effects on hippocampal and cerebellar LTD, although the mechanisms responsible for this difference remain largely unclear. Activity-dependent dephosphorylation of stargazin (an AMPA receptor auxiliary protein) by calcineurin regulates hippocampal LTD, but whether and how stargazin is involved in cerebellar LTD is unknown. In this study, we showed that stargazin is highly phosphorylated at basal states and is dephosphorylated by the application of high KCl plus glutamate (K-glu) or of a metabotropic glutamate receptor agonist, (S)-3,5-dihydroxyphenylglycine (DHPG), both of which chemically induced LTD in cerebellar slices. This chemically induced dephosphorylation of stargazin was specifically blocked by a calcineurin inhibitor. Indeed, inclusion of the calcineurin auto-inhibitory peptide in the patch pipette solution completely inhibited the LTD induced by the conjunctive stimulation of PFs and Purkinje cells. Furthermore, in Purkinje cells expressing stargazin-9D, in which all nine serine residues are mutated to aspartate, neither conjunctive stimulus nor DHPG treatment induced LTD. Finally, immunohistochemical analyses revealed that neither K-glu nor DHPG induced the endocytosis of AMPA receptors in Purkinje cells expressing stargazin-9D. Together, these results indicate that hippocampal and cerebellar LTD share a common pathway, namely dephosphorylation of stargazin by calcineurin.

Original languageEnglish
Pages (from-to)402-410
Number of pages9
JournalEuropean Journal of Neuroscience
Volume35
Issue number3
DOIs
Publication statusPublished - 2012 Feb

Fingerprint

Purkinje Cells
Depression
Calcineurin
AMPA Receptors
Endocytosis
Glutamic Acid
Isoxazoles
Excitatory Amino Acid Agonists
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Metabotropic Glutamate Receptors
TARP
Propionates
Phosphoric Monoester Hydrolases
Aspartic Acid
Hydroxyl Radical
Synapses
Serine
Phosphotransferases
Learning
Peptides

Keywords

  • Calcineurin
  • Cerebellum
  • Long-term depression
  • Mouse
  • Stargazin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cerebellar long-term depression requires dephosphorylation of TARP in Purkinje cells. / Nomura, Toshihiro; Kakegawa, Wataru; Matsuda, Shinji; Kohda, Kazuhisa; Nishiyama, Jun; Takahashi, Takao; Yuzaki, Michisuke.

In: European Journal of Neuroscience, Vol. 35, No. 3, 02.2012, p. 402-410.

Research output: Contribution to journalArticle

Nomura, Toshihiro ; Kakegawa, Wataru ; Matsuda, Shinji ; Kohda, Kazuhisa ; Nishiyama, Jun ; Takahashi, Takao ; Yuzaki, Michisuke. / Cerebellar long-term depression requires dephosphorylation of TARP in Purkinje cells. In: European Journal of Neuroscience. 2012 ; Vol. 35, No. 3. pp. 402-410.
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