Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils

Shigeaki Suzuki; Kortaro Tanaka; Eiichiro Nagata; Daisuke Ito; Tomohisa Dembo; Yasuo Fukuuchi

doi:10.1016/S0304-3940(99)00051-8

Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils

Shigeaki Suzuki, Kortaro Tanaka, Eiichiro Nagata, Daisuke Ito, Tomohisa Dembo, Yasuo Fukuuchi

Department of Internal Medicine (Neurology)

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Markedly increased interleukin-6 (IL-6) mRNA levels occur in experimental cerebral ischemia, although the protein production and cellular sources of IL-6 remain unclear. We examined the cellular localization of IL- 6 protein in gerbil brain following transient forebrain ischemia employing immunohistochemistry and Western blot analysis. The ischemia/recirculation groups revealed distinct IL-6 immunoreactivity predominantly in cortical and hippocampal neurons after 3 hours to 3 days recirculation. At 12 h recirculation, the IL-6 expression declined specifically in the hippocampus CA1. Microglia, but not activated astrocytes, also expressed IL-6 immunoreactivity. The sham group showed no apparent immunoreactivity. IL-6 protein may thus be expressed mainly in neurons following transient forebrain ischemia. Its transient decline in the CA1 at 12 h recirculation could reflect the specific vulnerability of this region.

Original language	English
Pages (from-to)	117-120
Number of pages	4
Journal	Neuroscience Letters
Volume	262
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(99)00051-8
Publication status	Published - 1999 Mar 5

Keywords

Cerebral cortex
Gerbil
Hippocampus
Immunohistochemistry
Interleukin- 6
Ischemia
Neuron
Western blot

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(99)00051-8

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title = "Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils",

abstract = "Markedly increased interleukin-6 (IL-6) mRNA levels occur in experimental cerebral ischemia, although the protein production and cellular sources of IL-6 remain unclear. We examined the cellular localization of IL- 6 protein in gerbil brain following transient forebrain ischemia employing immunohistochemistry and Western blot analysis. The ischemia/recirculation groups revealed distinct IL-6 immunoreactivity predominantly in cortical and hippocampal neurons after 3 hours to 3 days recirculation. At 12 h recirculation, the IL-6 expression declined specifically in the hippocampus CA1. Microglia, but not activated astrocytes, also expressed IL-6 immunoreactivity. The sham group showed no apparent immunoreactivity. IL-6 protein may thus be expressed mainly in neurons following transient forebrain ischemia. Its transient decline in the CA1 at 12 h recirculation could reflect the specific vulnerability of this region.",

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author = "Shigeaki Suzuki and Kortaro Tanaka and Eiichiro Nagata and Daisuke Ito and Tomohisa Dembo and Yasuo Fukuuchi",

year = "1999",

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T1 - Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils

AU - Suzuki, Shigeaki

AU - Tanaka, Kortaro

AU - Nagata, Eiichiro

AU - Ito, Daisuke

AU - Dembo, Tomohisa

AU - Fukuuchi, Yasuo

PY - 1999/3/5

Y1 - 1999/3/5

N2 - Markedly increased interleukin-6 (IL-6) mRNA levels occur in experimental cerebral ischemia, although the protein production and cellular sources of IL-6 remain unclear. We examined the cellular localization of IL- 6 protein in gerbil brain following transient forebrain ischemia employing immunohistochemistry and Western blot analysis. The ischemia/recirculation groups revealed distinct IL-6 immunoreactivity predominantly in cortical and hippocampal neurons after 3 hours to 3 days recirculation. At 12 h recirculation, the IL-6 expression declined specifically in the hippocampus CA1. Microglia, but not activated astrocytes, also expressed IL-6 immunoreactivity. The sham group showed no apparent immunoreactivity. IL-6 protein may thus be expressed mainly in neurons following transient forebrain ischemia. Its transient decline in the CA1 at 12 h recirculation could reflect the specific vulnerability of this region.

AB - Markedly increased interleukin-6 (IL-6) mRNA levels occur in experimental cerebral ischemia, although the protein production and cellular sources of IL-6 remain unclear. We examined the cellular localization of IL- 6 protein in gerbil brain following transient forebrain ischemia employing immunohistochemistry and Western blot analysis. The ischemia/recirculation groups revealed distinct IL-6 immunoreactivity predominantly in cortical and hippocampal neurons after 3 hours to 3 days recirculation. At 12 h recirculation, the IL-6 expression declined specifically in the hippocampus CA1. Microglia, but not activated astrocytes, also expressed IL-6 immunoreactivity. The sham group showed no apparent immunoreactivity. IL-6 protein may thus be expressed mainly in neurons following transient forebrain ischemia. Its transient decline in the CA1 at 12 h recirculation could reflect the specific vulnerability of this region.

KW - Cerebral cortex

KW - Gerbil

KW - Hippocampus

KW - Immunohistochemistry

KW - Interleukin- 6

KW - Ischemia

KW - Neuron

KW - Western blot

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ER -

Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils

Abstract

Keywords

ASJC Scopus subject areas

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