Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils

Shigeaki Suzuki, Kortaro Tanaka, Eiichiro Nagata, Daisuke Ito, Tomohisa Dembo, Yasuo Fukuuchi

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Markedly increased interleukin-6 (IL-6) mRNA levels occur in experimental cerebral ischemia, although the protein production and cellular sources of IL-6 remain unclear. We examined the cellular localization of IL- 6 protein in gerbil brain following transient forebrain ischemia employing immunohistochemistry and Western blot analysis. The ischemia/recirculation groups revealed distinct IL-6 immunoreactivity predominantly in cortical and hippocampal neurons after 3 hours to 3 days recirculation. At 12 h recirculation, the IL-6 expression declined specifically in the hippocampus CA1. Microglia, but not activated astrocytes, also expressed IL-6 immunoreactivity. The sham group showed no apparent immunoreactivity. IL-6 protein may thus be expressed mainly in neurons following transient forebrain ischemia. Its transient decline in the CA1 at 12 h recirculation could reflect the specific vulnerability of this region.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalNeuroscience Letters
Volume262
Issue number2
DOIs
Publication statusPublished - 1999 Mar 5

Keywords

  • Cerebral cortex
  • Gerbil
  • Hippocampus
  • Immunohistochemistry
  • Interleukin- 6
  • Ischemia
  • Neuron
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)

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