TY - JOUR
T1 - Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians
AU - Miura, Yuri
AU - Hashii, Noritaka
AU - Tsumoto, Hiroki
AU - Takakura, Daisuke
AU - Ohta, Yuki
AU - Abe, Yukiko
AU - Arai, Yasumichi
AU - Kawasaki, Nana
AU - Hirose, Nobuyoshi
AU - Endo, Tamao
N1 - Funding Information:
The Japan Society for the Promotion of Science (No. 24659141 to TE) (http://www.jsps.go.jp/ j-grantsinaid/index.html), and Mitsui Sumitomo Insurance Welfare Foundation (No. 18 to YM)(http://www.ms-ins.com/welfare/): The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank all the members of SONIC for their thoughtful advice and helpful discussions, and Dr. Yoshikawa (Infocom Co.) for technical assistance with O-PLS. The members of SONIC are: Dr. Yasumichi Arai (Keio University School of Medicine), Dr. Yasuyuki Gondo (Osaka University Graduate School of Human Sciences), Dr. Kazunori Ikebe (Osaka University Graduate School of Dentistry), Dr. Tatsuro Ishizaki (Tokyo Metropolitan Institute of Gerontology), Dr. Kei Kamide (Osaka University School of Medicine), Dr. Yukie Masui (Tokyo Metropolitan Institute of Gerontology), and Dr. Ryutaro Takahashi (Tokyo Metropolitan Institute of Gerontology).
Publisher Copyright:
© 2015 Miura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-á, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.
AB - An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-á, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.
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U2 - 10.1371/journal.pone.0142645
DO - 10.1371/journal.pone.0142645
M3 - Article
C2 - 26559536
AN - SCOPUS:84955494519
SN - 1932-6203
VL - 10
JO - PLoS One
JF - PLoS One
IS - 11
M1 - e0142645
ER -