Changes in atrial natriuretic peptide and brain natriuretic peptide associated with hypobaric hypoxia-induced pulmonary hypertension in rats

Kuniaki Nakanishi, Fumiko Tajima, Hiroshi Itoh, Yasuko Nakata, Hiroshi Osada, Norio Hama, Osamu Nakagawa, Kazuwa Nakao, Toshiaki Kawai, Kunio Takishima, Takashi Aurues, Tomosumi Ikeda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Experimental pulmonary hypertension induced in a hypobaric hypoxic environment (HHE) is characterized by structural remodeling of the heart and pulmonary arteries. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) both have diuretic, natriuretic, and hypotensive effects, and both are involved in cardiovascular homeostasis as cardiac hormones. To study the effects of HHE on the natriuretic peptide synthesis system, 170 male Wistar rats were housed in a chamber at the equivalent of the 5500-m altitude level for 1-12 weeks. After 1 week of HHE, pulmonary arterial pressure was significantly raised, and the ratio of left ventricle plus septum over right ventricle of the heart showed a significant decrease (compared with those of ground-level control rats). In both ventricular tissues, the expression of ANP messenger (m)RNA and BNP mRNA increased after exposure to HHE. The amounts of ANP and BNP had decreased significantly in right atrial tissue at 12 weeks of HHE (compared with those of the controls), whereas in ventricular tissues at the same time point, both levels had increased significantly. In in situ hybridization and immunohistochemical studies, the staining of the mRNAs for ANP and BNP and of ANP and BNP themselves was more intense in both ventricular tissues after exposure to HHE than before (i.e., in the controls). The results suggest that, in response to HHE, the changes in ventricular synthesis are similar for ANP and BNP. These changes may play a role in modulating pulmonary hypertension in HHE. However, under our conditions, pulmonary hypertension increased progressively throughout the HHE period.

Original languageEnglish
Pages (from-to)808-817
Number of pages10
JournalVirchows Archiv
Volume439
Issue number6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Atrial Natriuretic Factor
Pulmonary Hypertension
Messenger RNA
Heart Ventricles
Natriuretic Agents
Hypoxia
Natriuretic Peptides
Diuretics
Pulmonary Artery
In Situ Hybridization
Wistar Rats
Arterial Pressure
Homeostasis
Hormones
Staining and Labeling
Lung

Keywords

  • Hypoxia
  • Natriuretic peptide
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Changes in atrial natriuretic peptide and brain natriuretic peptide associated with hypobaric hypoxia-induced pulmonary hypertension in rats. / Nakanishi, Kuniaki; Tajima, Fumiko; Itoh, Hiroshi; Nakata, Yasuko; Osada, Hiroshi; Hama, Norio; Nakagawa, Osamu; Nakao, Kazuwa; Kawai, Toshiaki; Takishima, Kunio; Aurues, Takashi; Ikeda, Tomosumi.

In: Virchows Archiv, Vol. 439, No. 6, 2001, p. 808-817.

Research output: Contribution to journalArticle

Nakanishi, K, Tajima, F, Itoh, H, Nakata, Y, Osada, H, Hama, N, Nakagawa, O, Nakao, K, Kawai, T, Takishima, K, Aurues, T & Ikeda, T 2001, 'Changes in atrial natriuretic peptide and brain natriuretic peptide associated with hypobaric hypoxia-induced pulmonary hypertension in rats', Virchows Archiv, vol. 439, no. 6, pp. 808-817.
Nakanishi, Kuniaki ; Tajima, Fumiko ; Itoh, Hiroshi ; Nakata, Yasuko ; Osada, Hiroshi ; Hama, Norio ; Nakagawa, Osamu ; Nakao, Kazuwa ; Kawai, Toshiaki ; Takishima, Kunio ; Aurues, Takashi ; Ikeda, Tomosumi. / Changes in atrial natriuretic peptide and brain natriuretic peptide associated with hypobaric hypoxia-induced pulmonary hypertension in rats. In: Virchows Archiv. 2001 ; Vol. 439, No. 6. pp. 808-817.
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AU - Nakata, Yasuko

AU - Osada, Hiroshi

AU - Hama, Norio

AU - Nakagawa, Osamu

AU - Nakao, Kazuwa

AU - Kawai, Toshiaki

AU - Takishima, Kunio

AU - Aurues, Takashi

AU - Ikeda, Tomosumi

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