Changes in bacterial community structure associated with phytoplankton succession in outdoor experimental ponds

Yuki Kobayashi, Yoshikuni Hodoki, Kako Ohbayashi, Noboru Okuda, Shin Ichi Nakano

Research output: Contribution to journalArticle

Abstract

Changes in bacterial community structure were followed in two outdoor experimental ponds. To compare the changes in bacterial community, Microcystis was inoculated into one of the ponds but not to the other one. In both ponds, Chlorophyceae algae from the genera Cosmarium and Scenedesmus were dominant in the first and last months of the study. During the middle period of the study, cyanobacteria were dominant. Microcystis and Aphanizomenon dominated in one pond, and Planktothrix dominated in the other. To investigate bacterial phylogenetic abundance and these compositions, we used catalyzed reporter deposition fluorescence in situ hybridization (CARDFISH) and denaturing gradient gel electrophoresis (DGGE). A significant relationship was observed between the number of α-proteobacterial operational taxonomic units (OTUs) and the abundance of Chlorophyceae algae (p < 0.001), although no significant relationship was identified between the abundances of the two groups. The sequencing analysis of DGGE bands detected microcystin-degrading bacteria belonging to the α-proteobacteria as one of the dominant bacterial phylogenetic groups when Microcystis was the dominant phytoplankton. To our knowledge, this is the first report demonstrating changes in the abundance and composition of bacterial groups during the wax and wane of dominant phytoplankton taxa, using two different molecular methods.

Original languageEnglish
Pages (from-to)34-44
Number of pages11
JournalPlankton and Benthos Research
Volume10
Issue number1
DOIs
Publication statusPublished - 2015 Mar 20

Fingerprint

bacterial communities
algae
Microcystis
community structure
pond
phytoplankton
Chlorophyceae
denaturing gradient gel electrophoresis
electrokinesis
gel
alga
Planktothrix
Cosmarium
Aphanizomenon
phylogenetics
microcystins
Scenedesmus
Proteobacteria
phylogeny
wax

Keywords

  • Catalyzed reporter deposition fluorescence in situ hybridization
  • Chlorophyta
  • Cyanophyta
  • Denaturing gradient gel electrophoresis
  • Microcystis aeruginosa

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Changes in bacterial community structure associated with phytoplankton succession in outdoor experimental ponds. / Kobayashi, Yuki; Hodoki, Yoshikuni; Ohbayashi, Kako; Okuda, Noboru; Nakano, Shin Ichi.

In: Plankton and Benthos Research, Vol. 10, No. 1, 20.03.2015, p. 34-44.

Research output: Contribution to journalArticle

Kobayashi, Yuki ; Hodoki, Yoshikuni ; Ohbayashi, Kako ; Okuda, Noboru ; Nakano, Shin Ichi. / Changes in bacterial community structure associated with phytoplankton succession in outdoor experimental ponds. In: Plankton and Benthos Research. 2015 ; Vol. 10, No. 1. pp. 34-44.
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