Changes in Cell Membrane and Cellular Lipids in Apoptotic Cells Induced by Dolichyl Phosphate Differ from Findings in Cells Induced by Etoposide

Akiko Horiuchi, Etsuko Yasugi, Chizu Iwasaki, Keiji Fujimoto, Mieko Oshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell membrane fluidity and changes of cellular lipids of U937 cells during apoptosis are compared between the cells treated with dolichyl phosphate (dol-P) and etoposide. Dol-P induced lateral cell membrane perturbation immediately after addition and continued for at least 40 min. However, etoposide induced apoptosis without increase in cell membrane fluidity. Phosphatidylethanolamine was translocated together with phosphatidylserine after 4 hr of treatment in both dol-P and etoposide treated cells. The concentration of lipid levels changed in dol-P-induced cells and the cholesterol/phospholipid ratio almost doubled. Conversely, none of the phospholipid and cholesterol levels changed in etoposide treated cells. Although both dol-P and etoposide induce apoptosis, dol-P, but not etoposide, appeared to function as a modulator of membrane fluidity and to change membrane constituents and membrane physiology during the course of apoptosis.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalJournal of oleo science
Volume51
Issue number1
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • apoptosis
  • cholesterol
  • dolichyl phosphate
  • etoposide
  • membrane fluidity
  • phospholipid

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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