Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy

Yosuke Tajima, Hiroyuki Takuwa, Daisuke Kokuryo, Hiroshi Kawaguchi, Chie Seki, Kazuto Masamoto, Yoko Ikoma, Junko Taniguchi, Ichio Aoki, Yutaka Tomita, Norihiro Suzuki, Iwao Kanno, Naokatsu Saeki, Hiroshi Ito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study aimed to examine the cortical microvessel diameter response to hypercapnia in misery perfusion using two-photon laser scanning microscopy (TPLSM). We evaluated whether the vascular response to hypercapnia could represent the cerebrovascular reserve. Cerebral blood flow (CBF) during normocapnia and hypercapnia was measured by laser-Doppler flowmetry through cranial windows in awake C57/BL6 mice before and at 1, 7, 14, and 28 days after unilateral common carotid artery occlusion (UCCAO). Diameters of the cortical microvessels during normocapnia and hypercapnia were also measured by TPLSM. Cerebral blood flow and the vascular response to hypercapnia were decreased after UCCAO. Before UCCAO, vasodilation during hypercapnia was found primarily in arterioles (22.9%±3.5%). At 14 days after UCCAO, arterioles, capillaries, and venules were autoregulatorily dilated by 79.5%±19.7%, 57.2%±32.3%, and 32.0%±10.8%, respectively. At the same time, the diameter response to hypercapnia in arterioles was significantly decreased to 1.9%±1.5%. A significant negative correlation was observed between autoregulatory vasodilation and the diameter response to hypercapnia in arterioles. Our findings indicate that arterioles play main roles in both autoregulatory vasodilation and hypercapnic vasodilation, and that the vascular response to hypercapnia can be used to estimate the cerebrovascular reserve.

Original languageEnglish
Pages (from-to)1363-1372
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume34
Issue number8
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Hypercapnia
Microvessels
Photons
Confocal Microscopy
Perfusion
Arterioles
Common Carotid Artery
Vasodilation
Cerebrovascular Circulation
Blood Vessels
Laser-Doppler Flowmetry
Venules

Keywords

  • cerebral blood flow
  • cerebrovascular reserve
  • misery perfusion
  • two-photon laser scanning microscopy
  • vascular response

ASJC Scopus subject areas

  • Neurology
  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology

Cite this

Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy. / Tajima, Yosuke; Takuwa, Hiroyuki; Kokuryo, Daisuke; Kawaguchi, Hiroshi; Seki, Chie; Masamoto, Kazuto; Ikoma, Yoko; Taniguchi, Junko; Aoki, Ichio; Tomita, Yutaka; Suzuki, Norihiro; Kanno, Iwao; Saeki, Naokatsu; Ito, Hiroshi.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 34, No. 8, 2014, p. 1363-1372.

Research output: Contribution to journalArticle

Tajima, Y, Takuwa, H, Kokuryo, D, Kawaguchi, H, Seki, C, Masamoto, K, Ikoma, Y, Taniguchi, J, Aoki, I, Tomita, Y, Suzuki, N, Kanno, I, Saeki, N & Ito, H 2014, 'Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy', Journal of Cerebral Blood Flow and Metabolism, vol. 34, no. 8, pp. 1363-1372. https://doi.org/10.1038/jcbfm.2014.91
Tajima, Yosuke ; Takuwa, Hiroyuki ; Kokuryo, Daisuke ; Kawaguchi, Hiroshi ; Seki, Chie ; Masamoto, Kazuto ; Ikoma, Yoko ; Taniguchi, Junko ; Aoki, Ichio ; Tomita, Yutaka ; Suzuki, Norihiro ; Kanno, Iwao ; Saeki, Naokatsu ; Ito, Hiroshi. / Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy. In: Journal of Cerebral Blood Flow and Metabolism. 2014 ; Vol. 34, No. 8. pp. 1363-1372.
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abstract = "This study aimed to examine the cortical microvessel diameter response to hypercapnia in misery perfusion using two-photon laser scanning microscopy (TPLSM). We evaluated whether the vascular response to hypercapnia could represent the cerebrovascular reserve. Cerebral blood flow (CBF) during normocapnia and hypercapnia was measured by laser-Doppler flowmetry through cranial windows in awake C57/BL6 mice before and at 1, 7, 14, and 28 days after unilateral common carotid artery occlusion (UCCAO). Diameters of the cortical microvessels during normocapnia and hypercapnia were also measured by TPLSM. Cerebral blood flow and the vascular response to hypercapnia were decreased after UCCAO. Before UCCAO, vasodilation during hypercapnia was found primarily in arterioles (22.9{\%}±3.5{\%}). At 14 days after UCCAO, arterioles, capillaries, and venules were autoregulatorily dilated by 79.5{\%}±19.7{\%}, 57.2{\%}±32.3{\%}, and 32.0{\%}±10.8{\%}, respectively. At the same time, the diameter response to hypercapnia in arterioles was significantly decreased to 1.9{\%}±1.5{\%}. A significant negative correlation was observed between autoregulatory vasodilation and the diameter response to hypercapnia in arterioles. Our findings indicate that arterioles play main roles in both autoregulatory vasodilation and hypercapnic vasodilation, and that the vascular response to hypercapnia can be used to estimate the cerebrovascular reserve.",
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AU - Takuwa, Hiroyuki

AU - Kokuryo, Daisuke

AU - Kawaguchi, Hiroshi

AU - Seki, Chie

AU - Masamoto, Kazuto

AU - Ikoma, Yoko

AU - Taniguchi, Junko

AU - Aoki, Ichio

AU - Tomita, Yutaka

AU - Suzuki, Norihiro

AU - Kanno, Iwao

AU - Saeki, Naokatsu

AU - Ito, Hiroshi

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N2 - This study aimed to examine the cortical microvessel diameter response to hypercapnia in misery perfusion using two-photon laser scanning microscopy (TPLSM). We evaluated whether the vascular response to hypercapnia could represent the cerebrovascular reserve. Cerebral blood flow (CBF) during normocapnia and hypercapnia was measured by laser-Doppler flowmetry through cranial windows in awake C57/BL6 mice before and at 1, 7, 14, and 28 days after unilateral common carotid artery occlusion (UCCAO). Diameters of the cortical microvessels during normocapnia and hypercapnia were also measured by TPLSM. Cerebral blood flow and the vascular response to hypercapnia were decreased after UCCAO. Before UCCAO, vasodilation during hypercapnia was found primarily in arterioles (22.9%±3.5%). At 14 days after UCCAO, arterioles, capillaries, and venules were autoregulatorily dilated by 79.5%±19.7%, 57.2%±32.3%, and 32.0%±10.8%, respectively. At the same time, the diameter response to hypercapnia in arterioles was significantly decreased to 1.9%±1.5%. A significant negative correlation was observed between autoregulatory vasodilation and the diameter response to hypercapnia in arterioles. Our findings indicate that arterioles play main roles in both autoregulatory vasodilation and hypercapnic vasodilation, and that the vascular response to hypercapnia can be used to estimate the cerebrovascular reserve.

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