Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

Griselda Vallejo, Darío Maschi, Ana C. Mestre-Citrinovitz, Kazuhiro Aiba, Ricardo Maronna, Victor Yohai, Minoru Ko, Miguel Beato, Patricia Saragüeta

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

Original languageEnglish
Pages (from-to)127-137
Number of pages11
JournalJournal of Cellular Physiology
Volume222
Issue number1
DOIs
Publication statusPublished - 2010 Jan
Externally publishedYes

Fingerprint

Stromal Cells
Gene expression
Rats
Genes
Gene Expression
Decidua
Pregnancy
Signal transduction
Chromatin Assembly and Disassembly
Gene Regulatory Networks
Cell proliferation
Microarrays
Oligonucleotide Array Sequence Analysis
Cell culture
Chromatin
Signal Transduction
Proteins
Complementary DNA
Cell Culture Techniques
Cell Proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Vallejo, G., Maschi, D., Mestre-Citrinovitz, A. C., Aiba, K., Maronna, R., Yohai, V., ... Saragüeta, P. (2010). Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells. Journal of Cellular Physiology, 222(1), 127-137. https://doi.org/10.1002/jcp.21929

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells. / Vallejo, Griselda; Maschi, Darío; Mestre-Citrinovitz, Ana C.; Aiba, Kazuhiro; Maronna, Ricardo; Yohai, Victor; Ko, Minoru; Beato, Miguel; Saragüeta, Patricia.

In: Journal of Cellular Physiology, Vol. 222, No. 1, 01.2010, p. 127-137.

Research output: Contribution to journalArticle

Vallejo, G, Maschi, D, Mestre-Citrinovitz, AC, Aiba, K, Maronna, R, Yohai, V, Ko, M, Beato, M & Saragüeta, P 2010, 'Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells', Journal of Cellular Physiology, vol. 222, no. 1, pp. 127-137. https://doi.org/10.1002/jcp.21929
Vallejo, Griselda ; Maschi, Darío ; Mestre-Citrinovitz, Ana C. ; Aiba, Kazuhiro ; Maronna, Ricardo ; Yohai, Victor ; Ko, Minoru ; Beato, Miguel ; Saragüeta, Patricia. / Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells. In: Journal of Cellular Physiology. 2010 ; Vol. 222, No. 1. pp. 127-137.
@article{7001311d18e04072b76746656ed14a78,
title = "Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells",
abstract = "During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.",
author = "Griselda Vallejo and Dar{\'i}o Maschi and Mestre-Citrinovitz, {Ana C.} and Kazuhiro Aiba and Ricardo Maronna and Victor Yohai and Minoru Ko and Miguel Beato and Patricia Sarag{\"u}eta",
year = "2010",
month = "1",
doi = "10.1002/jcp.21929",
language = "English",
volume = "222",
pages = "127--137",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

AU - Vallejo, Griselda

AU - Maschi, Darío

AU - Mestre-Citrinovitz, Ana C.

AU - Aiba, Kazuhiro

AU - Maronna, Ricardo

AU - Yohai, Victor

AU - Ko, Minoru

AU - Beato, Miguel

AU - Saragüeta, Patricia

PY - 2010/1

Y1 - 2010/1

N2 - During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

AB - During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

UR - http://www.scopus.com/inward/record.url?scp=73949094304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73949094304&partnerID=8YFLogxK

U2 - 10.1002/jcp.21929

DO - 10.1002/jcp.21929

M3 - Article

VL - 222

SP - 127

EP - 137

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -