Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells

Hiroki Kuroda, Hirofumi Sakumoto, Kei Kinoshita, Makoto Asashima

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.

Original languageEnglish
Pages (from-to)283-291
Number of pages9
JournalDevelopment Growth and Differentiation
Volume41
Issue number3
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Xenopus laevis
Adhesives
Embryonic Structures
Mesoderm
Notochord
Transforming Growth Factors
Amphibians
Epidermis
activin A

Keywords

  • Activin A
  • Adhesive property
  • Animal cap
  • Mesoderm-inducer
  • Xenopus laevis

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells. / Kuroda, Hiroki; Sakumoto, Hirofumi; Kinoshita, Kei; Asashima, Makoto.

In: Development Growth and Differentiation, Vol. 41, No. 3, 1999, p. 283-291.

Research output: Contribution to journalArticle

Kuroda, Hiroki ; Sakumoto, Hirofumi ; Kinoshita, Kei ; Asashima, Makoto. / Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells. In: Development Growth and Differentiation. 1999 ; Vol. 41, No. 3. pp. 283-291.
@article{6ce632be1cd34a3299337ffc79619f47,
title = "Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells",
abstract = "Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.",
keywords = "Activin A, Adhesive property, Animal cap, Mesoderm-inducer, Xenopus laevis",
author = "Hiroki Kuroda and Hirofumi Sakumoto and Kei Kinoshita and Makoto Asashima",
year = "1999",
doi = "10.1046/j.1440-169X.1999.413428.x",
language = "English",
volume = "41",
pages = "283--291",
journal = "Development, growth & differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells

AU - Kuroda, Hiroki

AU - Sakumoto, Hirofumi

AU - Kinoshita, Kei

AU - Asashima, Makoto

PY - 1999

Y1 - 1999

N2 - Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.

AB - Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.

KW - Activin A

KW - Adhesive property

KW - Animal cap

KW - Mesoderm-inducer

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=0033020725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033020725&partnerID=8YFLogxK

U2 - 10.1046/j.1440-169X.1999.413428.x

DO - 10.1046/j.1440-169X.1999.413428.x

M3 - Article

VL - 41

SP - 283

EP - 291

JO - Development, growth & differentiation

JF - Development, growth & differentiation

SN - 0012-1592

IS - 3

ER -