Abstract
Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 283-291 |
| Number of pages | 9 |
| Journal | Development Growth and Differentiation |
| Volume | 41 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Activin A
- Adhesive property
- Animal cap
- Mesoderm-inducer
- Xenopus laevis
ASJC Scopus subject areas
- Anatomy
- Developmental Biology
- Cell Biology
Cite this
Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells. / Kuroda, Hiroki; Sakumoto, Hirofumi; Kinoshita, Kei; Asashima, Makoto.
In: Development Growth and Differentiation, Vol. 41, No. 3, 1999, p. 283-291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells
AU - Kuroda, Hiroki
AU - Sakumoto, Hirofumi
AU - Kinoshita, Kei
AU - Asashima, Makoto
PY - 1999
Y1 - 1999
N2 - Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.
AB - Activin A is a member of the transforming growth factor β superfamily, and the strongest candidate mesoderm-inducer. The initial adhesive property changes in amphibians are likely to be mediated by mesoderm-inducers like activin A. The manner in which these changes actually occur, however, remains poorly understood. In the present study, the adhesive property changes mediated by activin A were directly demonstrated. Activin A functioned as a morphogen at low concentrations (less than 0.5 ng/mL), with no effect on the type A adhesive property. But at high concentrations (1 ng/mL), it induced another type of adhesive property, type N, and at very high concentrations (more than 10 ng/mL), it induced yet another type of adhesive property, type Y. Cells that have types A, N, and Y adhesive properties ultimately differentiated into atypical epidermis, notochord, and yolk-rich cells, respectively. It was also shown that these changes occurred between 5 and 10h after induction by activin A. The implications of these results for the relationship between the adhesive property acquired during early and later stages of differentiation are also discussed.
KW - Activin A
KW - Adhesive property
KW - Animal cap
KW - Mesoderm-inducer
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=0033020725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033020725&partnerID=8YFLogxK
U2 - 10.1046/j.1440-169X.1999.413428.x
DO - 10.1046/j.1440-169X.1999.413428.x
M3 - Article
C2 - 10400390
AN - SCOPUS:0033020725
VL - 41
SP - 283
EP - 291
JO - Development, growth & differentiation
JF - Development, growth & differentiation
SN - 0012-1592
IS - 3
ER -