Changes in the charged metabolite and sugar profiles of pasteurized and unpasteurized Japanese sake with storage

Masahiro Sugimoto, Miku Kaneko, Hiromi Onuma, Yasuko Sakaguchi, Masayo Mori, Shinobu Abe, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Japanese sake (rice wine) is commonly heat treated (pasteurized) to maintain its quality. In this study, temporal changes in the metabolite profiles of pasteurized and unpasteurized sake were investigated during storage. Metabolomic analyses were conducted for eight sets of pasteurized and unpasteurized sake obtained from single process batches stored at 8 or 20 °C for 0, 1, 2, or 4 months. Capillary electrophoresis time-of-flight mass spectrometry and liquid chromatography tandem mass spectrometry were used to obtain charged metabolite and sugar profiles, respectively. The total amino acid concentration decreased with storage, and the decrease was faster in pasteurized sake than in unpasteurized. The organic acid concentrations were relatively constant in both types of sake. Peptide and glucose concentrations increased and polysaccharide concentrations decreased in unpasteurized sake, while they were relatively constant in pasteurized sake. Rather than stabilizing the sake metabolite profile during storage, pasteurization results in characteristic changes compared to unpasteurized sake.

Original languageEnglish
Pages (from-to)2586-2593
Number of pages8
JournalJournal of agricultural and food chemistry
Issue number10
Publication statusPublished - 2012 Mar 14


  • LC-MS/MS
  • Metabolome
  • Metabolomics
  • Pasteurized
  • Rice wine
  • Sake
  • Unpasteurized

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


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