Changes in the cytoplasmic and vacuolar pH in intact cells of mung bean root-tips under high-NaCl stress at different external concentrations of Ca+ ions

Yoshiyuki Nakamura, Tomoko Ogawa, Kunihiro Kasamo, Makoto Sakata, Eiji Ohta

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The cytoplasmic pH and the vacuolar pH in root-tip cells of intact mung bean seedlings under high-NaCl stress were measured by in vivo 31P-nuclear magnetic resonance (31P-NMR) spectroscopy. When roots were incubated with high levels (100 mM) of NaCl at the control external concentration (0.5 mM) of Ca2+ ions, the vacuolar pH increased rapidly from 5.6 to 6.2 within 3 h, while the cytoplasmic pH only decreased by a mere 0.1 pH unit even after a 24-h incubation under high-NaCl conditions. The increase in vacuolar pH induced by the high-NaCl stress was diminished by an increase in the external concentration of Ca2+ ions from 0.5 mM to 5 mM. The intracellular concentration of Na+ ions in the root-tip cells increased dramatically upon perfusion of the root cells with 100 mM NaCl, and high external levels of Ca2+ ions also suppressed the in flow of Na+ ions into the cells. The vacuolar alkalization observed in salt-stressed roots may be related to the inhibition of an H+-translocating pyrophosphatase in the tonoplast, caused by the increase in the cytoplasmic concentration of Na+ ions. It is suggested that, although the vacuolar pH increased markedly under salt stress, the cytoplasmic pH was tightly regulated by some unidentified mechanisms, such as stimulation of the H+-translocating ATPase of the plasmalemma, in roots of mung bean under salt stress.

Original languageEnglish
Pages (from-to)849-858
Number of pages10
JournalPlant and Cell Physiology
Volume33
Issue number7
Publication statusPublished - 1992 Oct

Fingerprint

Mung Bean
Meristem
mung beans
root tips
Roots
Ions
ions
ion
Cell
Salt Stress
cells
Salts
salt
calcium
Tonoplast
salt stress
Nuclear Magnetic Resonance
ATP Synthase
Inorganic Pyrophosphatase
pyrophosphatases

Keywords

  • 31P-nuclear magnetic resonance
  • Ca2+ ions
  • Cytoplasmic pH
  • Mung bean (Vigna mungo) root
  • Salt stress
  • Vacuolar pH

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Changes in the cytoplasmic and vacuolar pH in intact cells of mung bean root-tips under high-NaCl stress at different external concentrations of Ca+ ions. / Nakamura, Yoshiyuki; Ogawa, Tomoko; Kasamo, Kunihiro; Sakata, Makoto; Ohta, Eiji.

In: Plant and Cell Physiology, Vol. 33, No. 7, 10.1992, p. 849-858.

Research output: Contribution to journalArticle

Nakamura, Yoshiyuki ; Ogawa, Tomoko ; Kasamo, Kunihiro ; Sakata, Makoto ; Ohta, Eiji. / Changes in the cytoplasmic and vacuolar pH in intact cells of mung bean root-tips under high-NaCl stress at different external concentrations of Ca+ ions. In: Plant and Cell Physiology. 1992 ; Vol. 33, No. 7. pp. 849-858.
@article{b9d760a5f39848b6b76a81ab73fa0258,
title = "Changes in the cytoplasmic and vacuolar pH in intact cells of mung bean root-tips under high-NaCl stress at different external concentrations of Ca+ ions",
abstract = "The cytoplasmic pH and the vacuolar pH in root-tip cells of intact mung bean seedlings under high-NaCl stress were measured by in vivo 31P-nuclear magnetic resonance (31P-NMR) spectroscopy. When roots were incubated with high levels (100 mM) of NaCl at the control external concentration (0.5 mM) of Ca2+ ions, the vacuolar pH increased rapidly from 5.6 to 6.2 within 3 h, while the cytoplasmic pH only decreased by a mere 0.1 pH unit even after a 24-h incubation under high-NaCl conditions. The increase in vacuolar pH induced by the high-NaCl stress was diminished by an increase in the external concentration of Ca2+ ions from 0.5 mM to 5 mM. The intracellular concentration of Na+ ions in the root-tip cells increased dramatically upon perfusion of the root cells with 100 mM NaCl, and high external levels of Ca2+ ions also suppressed the in flow of Na+ ions into the cells. The vacuolar alkalization observed in salt-stressed roots may be related to the inhibition of an H+-translocating pyrophosphatase in the tonoplast, caused by the increase in the cytoplasmic concentration of Na+ ions. It is suggested that, although the vacuolar pH increased markedly under salt stress, the cytoplasmic pH was tightly regulated by some unidentified mechanisms, such as stimulation of the H+-translocating ATPase of the plasmalemma, in roots of mung bean under salt stress.",
keywords = "31P-nuclear magnetic resonance, Ca2+ ions, Cytoplasmic pH, Mung bean (Vigna mungo) root, Salt stress, Vacuolar pH",
author = "Yoshiyuki Nakamura and Tomoko Ogawa and Kunihiro Kasamo and Makoto Sakata and Eiji Ohta",
year = "1992",
month = "10",
language = "English",
volume = "33",
pages = "849--858",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Changes in the cytoplasmic and vacuolar pH in intact cells of mung bean root-tips under high-NaCl stress at different external concentrations of Ca+ ions

AU - Nakamura, Yoshiyuki

AU - Ogawa, Tomoko

AU - Kasamo, Kunihiro

AU - Sakata, Makoto

AU - Ohta, Eiji

PY - 1992/10

Y1 - 1992/10

N2 - The cytoplasmic pH and the vacuolar pH in root-tip cells of intact mung bean seedlings under high-NaCl stress were measured by in vivo 31P-nuclear magnetic resonance (31P-NMR) spectroscopy. When roots were incubated with high levels (100 mM) of NaCl at the control external concentration (0.5 mM) of Ca2+ ions, the vacuolar pH increased rapidly from 5.6 to 6.2 within 3 h, while the cytoplasmic pH only decreased by a mere 0.1 pH unit even after a 24-h incubation under high-NaCl conditions. The increase in vacuolar pH induced by the high-NaCl stress was diminished by an increase in the external concentration of Ca2+ ions from 0.5 mM to 5 mM. The intracellular concentration of Na+ ions in the root-tip cells increased dramatically upon perfusion of the root cells with 100 mM NaCl, and high external levels of Ca2+ ions also suppressed the in flow of Na+ ions into the cells. The vacuolar alkalization observed in salt-stressed roots may be related to the inhibition of an H+-translocating pyrophosphatase in the tonoplast, caused by the increase in the cytoplasmic concentration of Na+ ions. It is suggested that, although the vacuolar pH increased markedly under salt stress, the cytoplasmic pH was tightly regulated by some unidentified mechanisms, such as stimulation of the H+-translocating ATPase of the plasmalemma, in roots of mung bean under salt stress.

AB - The cytoplasmic pH and the vacuolar pH in root-tip cells of intact mung bean seedlings under high-NaCl stress were measured by in vivo 31P-nuclear magnetic resonance (31P-NMR) spectroscopy. When roots were incubated with high levels (100 mM) of NaCl at the control external concentration (0.5 mM) of Ca2+ ions, the vacuolar pH increased rapidly from 5.6 to 6.2 within 3 h, while the cytoplasmic pH only decreased by a mere 0.1 pH unit even after a 24-h incubation under high-NaCl conditions. The increase in vacuolar pH induced by the high-NaCl stress was diminished by an increase in the external concentration of Ca2+ ions from 0.5 mM to 5 mM. The intracellular concentration of Na+ ions in the root-tip cells increased dramatically upon perfusion of the root cells with 100 mM NaCl, and high external levels of Ca2+ ions also suppressed the in flow of Na+ ions into the cells. The vacuolar alkalization observed in salt-stressed roots may be related to the inhibition of an H+-translocating pyrophosphatase in the tonoplast, caused by the increase in the cytoplasmic concentration of Na+ ions. It is suggested that, although the vacuolar pH increased markedly under salt stress, the cytoplasmic pH was tightly regulated by some unidentified mechanisms, such as stimulation of the H+-translocating ATPase of the plasmalemma, in roots of mung bean under salt stress.

KW - 31P-nuclear magnetic resonance

KW - Ca2+ ions

KW - Cytoplasmic pH

KW - Mung bean (Vigna mungo) root

KW - Salt stress

KW - Vacuolar pH

UR - http://www.scopus.com/inward/record.url?scp=0342940107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342940107&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0342940107

VL - 33

SP - 849

EP - 858

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 7

ER -