Changes in urinary titin N-terminal fragments as a biomarker of exercise-induced muscle damage in the repeated bout effect

Shota Yamaguchi, Katsuhiko Suzuki, Kazue Kanda, Takayuki Inami, Junichi Okada

Research output: Contribution to journalArticle

Abstract

Objectives: Muscle damage symptoms induced by unaccustomed eccentric contraction exercise can be reduced by repeating the experience several times. This phenomenon is termed the repeated bout effect. Although traditional biochemical markers require invasive blood sampling, biochemical measurements have recently been developed that can be non-invasively performed using urinary titin N-terminal fragment (UTF). However, it is unclear whether UTF can reflect the repeated bout effect. Therefore, the aim of the present study was to clarify whether UTF decreased with the repeated bout effect. Design: This study compared changes in muscle damage markers between bouts of exercise performed for the first and second time. Methods: Eight young men performed 30 eccentric exercises of the elbow flexor on the first day of the first week (Bout 1). A second bout of eccentric exercises, same as the first, was performed 2 weeks later, (Bout 2). The dependent variables were muscle soreness (SOR), maximal voluntary isometric contraction (MVIC), range of motion (ROM), creatine kinase (CK), and UTF. All dependent variables were analyzed using two-way analysis of variance. Results: No significant difference was observed in workload or peak torque between the first and second exercise bouts. SOR as well as CK and UTF were significantly lower and ROM and MVIC were significantly higher in Bout 2 in comparison to Bout 1. Conclusions: These results suggest that UTF sensitively reflects the repeated bout effect and exercise-induced muscle damage can be non-invasively measured.

Original languageEnglish
JournalJournal of Science and Medicine in Sport
DOIs
Publication statusAccepted/In press - 2020 Jan 1

Fingerprint

Connectin
Biomarkers
Exercise
Muscles
Isometric Contraction
Creatine Kinase
Articular Range of Motion
Myalgia
Torque
Elbow
Workload
Analysis of Variance

Keywords

  • Creatine kinase
  • Delayed-onset muscle soreness
  • Eccentric contraction
  • Non-invasive
  • Urine

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Changes in urinary titin N-terminal fragments as a biomarker of exercise-induced muscle damage in the repeated bout effect. / Yamaguchi, Shota; Suzuki, Katsuhiko; Kanda, Kazue; Inami, Takayuki; Okada, Junichi.

In: Journal of Science and Medicine in Sport, 01.01.2020.

Research output: Contribution to journalArticle

@article{703b454e38764220aee25c398b8902a8,
title = "Changes in urinary titin N-terminal fragments as a biomarker of exercise-induced muscle damage in the repeated bout effect",
abstract = "Objectives: Muscle damage symptoms induced by unaccustomed eccentric contraction exercise can be reduced by repeating the experience several times. This phenomenon is termed the repeated bout effect. Although traditional biochemical markers require invasive blood sampling, biochemical measurements have recently been developed that can be non-invasively performed using urinary titin N-terminal fragment (UTF). However, it is unclear whether UTF can reflect the repeated bout effect. Therefore, the aim of the present study was to clarify whether UTF decreased with the repeated bout effect. Design: This study compared changes in muscle damage markers between bouts of exercise performed for the first and second time. Methods: Eight young men performed 30 eccentric exercises of the elbow flexor on the first day of the first week (Bout 1). A second bout of eccentric exercises, same as the first, was performed 2 weeks later, (Bout 2). The dependent variables were muscle soreness (SOR), maximal voluntary isometric contraction (MVIC), range of motion (ROM), creatine kinase (CK), and UTF. All dependent variables were analyzed using two-way analysis of variance. Results: No significant difference was observed in workload or peak torque between the first and second exercise bouts. SOR as well as CK and UTF were significantly lower and ROM and MVIC were significantly higher in Bout 2 in comparison to Bout 1. Conclusions: These results suggest that UTF sensitively reflects the repeated bout effect and exercise-induced muscle damage can be non-invasively measured.",
keywords = "Creatine kinase, Delayed-onset muscle soreness, Eccentric contraction, Non-invasive, Urine",
author = "Shota Yamaguchi and Katsuhiko Suzuki and Kazue Kanda and Takayuki Inami and Junichi Okada",
year = "2020",
month = "1",
day = "1",
doi = "10.1016/j.jsams.2019.12.023",
language = "English",
journal = "Journal of Science and Medicine in Sport",
issn = "1440-2440",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Changes in urinary titin N-terminal fragments as a biomarker of exercise-induced muscle damage in the repeated bout effect

AU - Yamaguchi, Shota

AU - Suzuki, Katsuhiko

AU - Kanda, Kazue

AU - Inami, Takayuki

AU - Okada, Junichi

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Objectives: Muscle damage symptoms induced by unaccustomed eccentric contraction exercise can be reduced by repeating the experience several times. This phenomenon is termed the repeated bout effect. Although traditional biochemical markers require invasive blood sampling, biochemical measurements have recently been developed that can be non-invasively performed using urinary titin N-terminal fragment (UTF). However, it is unclear whether UTF can reflect the repeated bout effect. Therefore, the aim of the present study was to clarify whether UTF decreased with the repeated bout effect. Design: This study compared changes in muscle damage markers between bouts of exercise performed for the first and second time. Methods: Eight young men performed 30 eccentric exercises of the elbow flexor on the first day of the first week (Bout 1). A second bout of eccentric exercises, same as the first, was performed 2 weeks later, (Bout 2). The dependent variables were muscle soreness (SOR), maximal voluntary isometric contraction (MVIC), range of motion (ROM), creatine kinase (CK), and UTF. All dependent variables were analyzed using two-way analysis of variance. Results: No significant difference was observed in workload or peak torque between the first and second exercise bouts. SOR as well as CK and UTF were significantly lower and ROM and MVIC were significantly higher in Bout 2 in comparison to Bout 1. Conclusions: These results suggest that UTF sensitively reflects the repeated bout effect and exercise-induced muscle damage can be non-invasively measured.

AB - Objectives: Muscle damage symptoms induced by unaccustomed eccentric contraction exercise can be reduced by repeating the experience several times. This phenomenon is termed the repeated bout effect. Although traditional biochemical markers require invasive blood sampling, biochemical measurements have recently been developed that can be non-invasively performed using urinary titin N-terminal fragment (UTF). However, it is unclear whether UTF can reflect the repeated bout effect. Therefore, the aim of the present study was to clarify whether UTF decreased with the repeated bout effect. Design: This study compared changes in muscle damage markers between bouts of exercise performed for the first and second time. Methods: Eight young men performed 30 eccentric exercises of the elbow flexor on the first day of the first week (Bout 1). A second bout of eccentric exercises, same as the first, was performed 2 weeks later, (Bout 2). The dependent variables were muscle soreness (SOR), maximal voluntary isometric contraction (MVIC), range of motion (ROM), creatine kinase (CK), and UTF. All dependent variables were analyzed using two-way analysis of variance. Results: No significant difference was observed in workload or peak torque between the first and second exercise bouts. SOR as well as CK and UTF were significantly lower and ROM and MVIC were significantly higher in Bout 2 in comparison to Bout 1. Conclusions: These results suggest that UTF sensitively reflects the repeated bout effect and exercise-induced muscle damage can be non-invasively measured.

KW - Creatine kinase

KW - Delayed-onset muscle soreness

KW - Eccentric contraction

KW - Non-invasive

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=85077707249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85077707249&partnerID=8YFLogxK

U2 - 10.1016/j.jsams.2019.12.023

DO - 10.1016/j.jsams.2019.12.023

M3 - Article

AN - SCOPUS:85077707249

JO - Journal of Science and Medicine in Sport

JF - Journal of Science and Medicine in Sport

SN - 1440-2440

ER -