Changes of corneal redox state in diabetic animal models

J. Shimazaki, Kazuo Tsubota, A. Yoshida, K. Tornheim, R. A. Laing

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Metabolic and biochemical changes of the corneal epithelium and endothelium were studied in experimental diabetic animal models. Ocular redox fluorometry was used to noninvasively determine tissue reduction-oxidation (redox) changes in organ cultured rabbit corneas incubated in high glucose concentration media, alloxan-induced diabetic rabbits, and nonobese diabetic mice. The ratio of autofluorescence from reduced pyridine nucleotide to oxidized flavoproteins (PN/Fp) was used as the indicator of the redox state. Chemical assays for NADH and NAD+ were performed on in vitro materials. Analysis of corneal endothelial morphology using specular microscopy was performed to study possible correlations with metabolic changes. Both the PN/Fp and NADH/NAD+ ratios increased in the corneal endothelium under all experimental conditions. Changes in redox state were not observed in the corneal epithelium in any of the models. Morphologic analysis of the corneal endothelium revealed no significant changes. These results indicate that redox changes occur in the diabetic corneal endothelium, but not the corneal epithelium. Ocular redox fluorometry is capable of detecting changes in the corneal endothelial redox state noninvasively.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalCornea
Volume14
Issue number2
Publication statusPublished - 1995
Externally publishedYes

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Corneal Endothelium
NAD
Corneal Epithelium
Animal Models
Fluorometry
Rabbits
Flavoproteins
Inbred NOD Mouse
Alloxan
Cornea
Microscopy
Nucleotides
Glucose

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Shimazaki, J., Tsubota, K., Yoshida, A., Tornheim, K., & Laing, R. A. (1995). Changes of corneal redox state in diabetic animal models. Cornea, 14(2), 196-201.

Changes of corneal redox state in diabetic animal models. / Shimazaki, J.; Tsubota, Kazuo; Yoshida, A.; Tornheim, K.; Laing, R. A.

In: Cornea, Vol. 14, No. 2, 1995, p. 196-201.

Research output: Contribution to journalArticle

Shimazaki, J, Tsubota, K, Yoshida, A, Tornheim, K & Laing, RA 1995, 'Changes of corneal redox state in diabetic animal models', Cornea, vol. 14, no. 2, pp. 196-201.
Shimazaki J, Tsubota K, Yoshida A, Tornheim K, Laing RA. Changes of corneal redox state in diabetic animal models. Cornea. 1995;14(2):196-201.
Shimazaki, J. ; Tsubota, Kazuo ; Yoshida, A. ; Tornheim, K. ; Laing, R. A. / Changes of corneal redox state in diabetic animal models. In: Cornea. 1995 ; Vol. 14, No. 2. pp. 196-201.
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