CHAPTER 7: CE-MS for Anionic and Cationic Metabolic Profiling: System Optimization and Applications

Akiyoshi Hirayama, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be an important technology for metabolomics studies. Most primary metabolite intermediates contain hydroxyl, amino, carboxyl and phosphate groups and, therefore, are charged, which makes them suitable for CE-MS analysis. The major advantages of CE-MS are its high resolution and the fact that almost any charged metabolite (both cationic and anionic) can be analyzed by only two analytical modes. CE-MS can be readily applied to various kinds of biological samples. This chapter highlights the general CE-MS conditions specifically developed for profiling cationic and anionic metabolites, and also focuses on several biological samples frequently used in medical and biochemical metabolomics, including blood (serum and plasma), urine, tissue, cultured cells and other related samples.

Original languageEnglish
Title of host publicationNew Developments in NMR
EditorsRawi Ramautar
PublisherRoyal Society of Chemistry
Pages134-160
Number of pages27
Edition6
ISBN (Electronic)9781782624479, 9781782628545, 9781849736435, 9781849739139
ISBN (Print)9781788010863, 9781788011044
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameNew Developments in NMR
Number6
Volume2018-January
ISSN (Print)2044-253X
ISSN (Electronic)2044-2548

ASJC Scopus subject areas

  • Analytical Chemistry
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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    Hirayama, A., & Soga, T. (2018). CHAPTER 7: CE-MS for Anionic and Cationic Metabolic Profiling: System Optimization and Applications. In R. Ramautar (Ed.), New Developments in NMR (6 ed., pp. 134-160). (New Developments in NMR; Vol. 2018-January, No. 6). Royal Society of Chemistry. https://doi.org/10.1039/9781788012737-00134