TY - JOUR
T1 - Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins
AU - Nakao, Mitsuyoshi
AU - Inanaga, Daimu
AU - Nagase, Kenichi
AU - Kanazawa, Hideko
N1 - Funding Information:
This work was partly financially supported by a SENTAN grant from the Japan Science and Technology Agency , a JSPS KAKENHI Grant No. JP16H05083 and JP18K19938 from the Japan Society for the Promotion of Science (JSPS), and research grant from Terumo Foundation for Life Science and Arts .
Publisher Copyright:
© 2019 The Japanese Society for Regenerative Medicine
PY - 2019/12
Y1 - 2019/12
N2 - Introduction: Stem cell therapy with mesenchymal stem cells (MSCs)has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated. Methods: Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse microscopy. Results: Different cell adhesion and proliferation rates in temperature-responsive cell culture dishes were observed among the three types of MSCs. FBS pre-coating of the dishes enhanced cell attachment and proliferation in all cell types. Harvested cell sheets showed high attachment capacity to tissue culture polystyrene dish surfaces. Conclusions: MSC sheets can be fabricated from MSCs from different tissue origins using temperature-responsive cell culture dishes. The fabricated MSC sheets could be useful in cell transplantation therapies by choosing appropriate types of MSCs that secrete therapeutic cytokines for the targeted diseases.
AB - Introduction: Stem cell therapy with mesenchymal stem cells (MSCs)has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated. Methods: Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse microscopy. Results: Different cell adhesion and proliferation rates in temperature-responsive cell culture dishes were observed among the three types of MSCs. FBS pre-coating of the dishes enhanced cell attachment and proliferation in all cell types. Harvested cell sheets showed high attachment capacity to tissue culture polystyrene dish surfaces. Conclusions: MSC sheets can be fabricated from MSCs from different tissue origins using temperature-responsive cell culture dishes. The fabricated MSC sheets could be useful in cell transplantation therapies by choosing appropriate types of MSCs that secrete therapeutic cytokines for the targeted diseases.
KW - Cell adhesion
KW - Cell sheet engineering
KW - Cytokine expression
KW - Mesenchymal stem cell
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U2 - 10.1016/j.reth.2019.01.002
DO - 10.1016/j.reth.2019.01.002
M3 - Article
AN - SCOPUS:85065226030
SN - 2352-3204
VL - 11
SP - 34
EP - 40
JO - Regenerative Therapy
JF - Regenerative Therapy
ER -