Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins

Mitsuyoshi Nakao, Daimu Inanaga, Kenichi Nagase, Hideko Kanazawa

Research output: Contribution to journalArticle

Abstract

Introduction: Stem cell therapy with mesenchymal stem cells (MSCs)has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated. Methods: Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse microscopy. Results: Different cell adhesion and proliferation rates in temperature-responsive cell culture dishes were observed among the three types of MSCs. FBS pre-coating of the dishes enhanced cell attachment and proliferation in all cell types. Harvested cell sheets showed high attachment capacity to tissue culture polystyrene dish surfaces. Conclusions: MSC sheets can be fabricated from MSCs from different tissue origins using temperature-responsive cell culture dishes. The fabricated MSC sheets could be useful in cell transplantation therapies by choosing appropriate types of MSCs that secrete therapeutic cytokines for the targeted diseases.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalRegenerative Therapy
Volume11
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Tissue
Cell culture
Cell Culture Techniques
Cell Proliferation
Cell adhesion
Cell- and Tissue-Based Therapy
Cell proliferation
Cell Adhesion
Temperature
Cytokines
Coatings
Tissue culture
Umbilical Cord
Polystyrenes
Cell Transplantation
Serum
Adipose Tissue
Microscopy

Keywords

  • Cell adhesion
  • Cell sheet engineering
  • Cytokine expression
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

Cite this

Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins. / Nakao, Mitsuyoshi; Inanaga, Daimu; Nagase, Kenichi; Kanazawa, Hideko.

In: Regenerative Therapy, Vol. 11, 01.12.2019, p. 34-40.

Research output: Contribution to journalArticle

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