Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast

Masako Inuyama, Fumihiko Ushigome, Akiko Emoto, Noriko Koyabu, Shoji Satoh, Kiyomi Tsukimori, Hitoo Nakano, Hisakazu Ohtani, Yasufumi Sawada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and α-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4′-diisothiocyanostilbene-2,2′-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number3 52-3
Publication statusPublished - 2002 Sep
Externally publishedYes

Fingerprint

Trophoblasts
Lactic Acid
Membranes
Brushes
Microvilli
Proton-Motive Force
Monocarboxylic Acid Transporters
4-Chloromercuribenzenesulfonate
Phloretin
Dicarboxylic Acids
Active Biological Transport
Placenta
Kinetics

Keywords

  • Human placenta
  • Monocarboxylic acid
  • Trophoblast

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Inuyama, M., Ushigome, F., Emoto, A., Koyabu, N., Satoh, S., Tsukimori, K., ... Sawada, Y. (2002). Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. American Journal of Physiology - Cell Physiology, 283(3 52-3).

Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. / Inuyama, Masako; Ushigome, Fumihiko; Emoto, Akiko; Koyabu, Noriko; Satoh, Shoji; Tsukimori, Kiyomi; Nakano, Hitoo; Ohtani, Hisakazu; Sawada, Yasufumi.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 3 52-3, 09.2002.

Research output: Contribution to journalArticle

Inuyama, M, Ushigome, F, Emoto, A, Koyabu, N, Satoh, S, Tsukimori, K, Nakano, H, Ohtani, H & Sawada, Y 2002, 'Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast', American Journal of Physiology - Cell Physiology, vol. 283, no. 3 52-3.
Inuyama, Masako ; Ushigome, Fumihiko ; Emoto, Akiko ; Koyabu, Noriko ; Satoh, Shoji ; Tsukimori, Kiyomi ; Nakano, Hitoo ; Ohtani, Hisakazu ; Sawada, Yasufumi. / Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 3 52-3.
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