Characteristics of photocytotoxicity with high peak power pulsed irradiation: Comparison of photodynamic therapy with two photosensitizers, photofrin® and mono-L-aspartyl chlorin e6 on prostate cancer cell in vitro

S. Ohmori, K. Masuda, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We proposed novel depth-controlled photodynamie therapy (PDT) by varying pulsed laser irradiation condition. We focus on photoeytotoxicity suppression under high peak power pulsed irradiation to apply this phenomenon to surface intact therapy to preserve healthy wall of a hollow organ. The relation between laser irradiation condition and cell lethality of rat prostate cancer cell with PDT using practical photosensitizers, mono-L-aspartyl chlorine e6 (ME2906) and Photofrin® was investigated. We found cell lethality suppression from 63 % to 20 % when the irradiated pulsed peak power density ranged from 0.2 to 1.4 mW/cm with ME2906 mediated PDT. There was no significant photoeytotoxicity suppression in case of Photofrin® mediated PDT. Singlet oxygen luminescence from the two different kinds of photosensitizer solution was measured. The pulse peak power dependence of singlet oxygen luminescence intensity correlated with the photoeytotoxicity. We think the photoeytotoxicity suppression with high peak power pulsed irradiation with ME2906 might be useful for the therapeutic depth controlled PDT without damage on the surface of a hollow organ.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages217-219
Number of pages3
Volume7 VOLS
Publication statusPublished - 2005
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: 2005 Sep 12005 Sep 4

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
CountryChina
CityShanghai
Period05/9/105/9/4

    Fingerprint

ASJC Scopus subject areas

  • Bioengineering

Cite this

Ohmori, S., Masuda, K., & Arai, T. (2005). Characteristics of photocytotoxicity with high peak power pulsed irradiation: Comparison of photodynamic therapy with two photosensitizers, photofrin® and mono-L-aspartyl chlorin e6 on prostate cancer cell in vitro. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 7 VOLS, pp. 217-219). [1616382]