Characterization and biological application of YAG

Ce3+ nanophosphor modified with mercaptopropyl trimethoxy silane

Atsushi Tsukamoto, Tetsuhiko Isobe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We focus on the inorganic nanophosphor of YAG:Ce3+ nanoparticles as an alternative fluorescent probe instead of organic dyes for biological application. YAG:Ce3+ nanoparticles have the green emission at 530 nm under the excitation of blue light at 450 nm. Conventional biochemical equipments for organic dyes can be used for YAG:Ce3+ nanoparticles. SH groups were introduced by surface modification of YAG:Ce3+ nanoparticles with 3-mercaptopropyl trimethoxy silane, and were characterized by X-ray fluorescence analysis, FT-IR, and Ellman method. We demonstrated tagging avidin-immobilized agarose gel beads with biotinylated-YAG:Ce3+ nanoparticles, and tagging rabbit IgG-immobilized agarose gel beads with antirabbit IgG-immobilized YAG:Ce3+ nanoparticles.

Original languageEnglish
Pages (from-to)1344-1350
Number of pages7
JournalJournal of Materials Science
Volume44
Issue number5
DOIs
Publication statusPublished - 2009 Mar

Fingerprint

Silanes
Nanoparticles
Sepharose
Coloring Agents
Gels
Dyes
Immunoglobulin G
Avidin
Fluorescent Dyes
Surface treatment
Fluorescence
X rays

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Characterization and biological application of YAG : Ce3+ nanophosphor modified with mercaptopropyl trimethoxy silane. / Tsukamoto, Atsushi; Isobe, Tetsuhiko.

In: Journal of Materials Science, Vol. 44, No. 5, 03.2009, p. 1344-1350.

Research output: Contribution to journalArticle

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