Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid

Zhi Hong Yang, Yoshiyuki Koyama, Toshinori Sato

Research output: Contribution to conferencePaper

Abstract

In this study, we investigated receptor-mediated transfection of the ternary nanoparticles in which a pDNA/chitosan particles is coated with hyaluronic acid (HA), that is known to specifically bind CD44. It was found that the coating of the pDNA/chiorsan complexes with HA depressed self-aggregation in BSA solution, and its cytotoxicity was very low. Although the ternary nanoparticles was negative charge, the gene expression of luciferase gene on CHO cells which express CD44 were improved, which is well agreed with the cell uptake efficiency.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

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Keywords

  • Chitosan
  • Gene transfer
  • Hayluronic acid/CD44

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Yang, Z. H., Koyama, Y., & Sato, T. (2006). Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.