Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasma pneumoniae ribosomal protein L7/L12 antigens

Go Sano, Tsutomu Itagaki, Naruhiko Ishiwada, Keita Matsubara, Satoshi Iwata, Yoshitaka Nakamori, Kenji Matsuyama, Katsuya Watanabe, Yoshikazu Ishii, Sakae Homma, Kazuhiro Tateda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Point-of-care testing for Mycoplasma pneumoniae infection may be ideal and useful because significant numbers of the cases will be seen as outpatients. Recently,a new immunochromatographic method (ICM) targeting M. pneumoniae ribosomal protein L7/L12 (RP-L7/L12) in pharyngeal swabs became available in Japan,although clinical data and basic information regarding efficacy and characterization of this ICM are limited. The present study examined the fate of M. pneumoniae RP-L7/L12 during in vitro growth and the correlation between M. pneumoniae concentration in clinical specimens and the sensitivity of the ICM test. The usefulness of the ICM was investigated in patients suspected of having M. pneumoniae pneumonia and upper respiratory tract infection (137 children and 39 adults). The limit of detection for the ICM test was 1.1×104 c.f.u. ml-1 of M. pneumoniae. Bacterial production of RP-L7/L12 correlated positively with the viable M. pneumoniae concentration in vitro; antigen was then degraded in culture broth,with an in vitro half-life of approximately 2 days. Five other Mycoplasma spp. and 14 representative respiratory pathogens were ICM assay negative at bacterial concentrations of 106 c.f.u. ml-1. The clinical sensitivity and specificity of the ICM assay were 57.1% (20/35) and 92.2% (130/141),respectively,in comparison with bacterial culture. Clinical specimens containing >106 c.f.u. ml-1 of M. pneumoniae burden were ICM positive in 13 of 18 cases (72.2 %). The ICM is a poorly sensitive but reasonably specific means for detecting M. pneumoniae infections.

Original languageEnglish
Pages (from-to)1105-1110
Number of pages6
JournalJournal of Medical Microbiology
Volume65
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1
Externally publishedYes

Fingerprint

Immunochromatography
Mycoplasma pneumoniae
Antigens
Mycoplasma Pneumonia
ribosomal protein L7-L12
Mycoplasma
Infection
Respiratory Tract Infections
Half-Life
Limit of Detection
Japan
Outpatients

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasma pneumoniae ribosomal protein L7/L12 antigens. / Sano, Go; Itagaki, Tsutomu; Ishiwada, Naruhiko; Matsubara, Keita; Iwata, Satoshi; Nakamori, Yoshitaka; Matsuyama, Kenji; Watanabe, Katsuya; Ishii, Yoshikazu; Homma, Sakae; Tateda, Kazuhiro.

In: Journal of Medical Microbiology, Vol. 65, No. 10, 01.10.2016, p. 1105-1110.

Research output: Contribution to journalArticle

Sano, G, Itagaki, T, Ishiwada, N, Matsubara, K, Iwata, S, Nakamori, Y, Matsuyama, K, Watanabe, K, Ishii, Y, Homma, S & Tateda, K 2016, 'Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasma pneumoniae ribosomal protein L7/L12 antigens', Journal of Medical Microbiology, vol. 65, no. 10, pp. 1105-1110. https://doi.org/10.1099/jmm.0.000336
Sano, Go ; Itagaki, Tsutomu ; Ishiwada, Naruhiko ; Matsubara, Keita ; Iwata, Satoshi ; Nakamori, Yoshitaka ; Matsuyama, Kenji ; Watanabe, Katsuya ; Ishii, Yoshikazu ; Homma, Sakae ; Tateda, Kazuhiro. / Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasma pneumoniae ribosomal protein L7/L12 antigens. In: Journal of Medical Microbiology. 2016 ; Vol. 65, No. 10. pp. 1105-1110.
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AU - Matsuyama, Kenji

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