Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails

Toshinori Sato, Hiroyuki Akino, Yoko Shoji, Jingoro Shimada, Yoshio Okahata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume9
Issue number1
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Keywords

  • Confocal laser scanning microscope
  • DNA
  • DNA complex
  • Gene transfer
  • Lipoglutamide
  • Poly(ethylene glycol)
  • Subcellular distribution

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails'. Together they form a unique fingerprint.

  • Cite this