Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails

Toshinori Sato; Hiroyuki Akino; Yoko Shoji; Jingoro Shimada; Yoshio Okahata

doi:10.1163/156856297X00245

Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails

Toshinori Sato, Hiroyuki Akino, Yoko Shoji, Jingoro Shimada, Yoshio Okahata

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.

Original language	English
Pages (from-to)	31-42
Number of pages	12
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	9
Issue number	1
DOIs	https://doi.org/10.1163/156856297X00245
Publication status	Published - 1997 Jan 1
Externally published	Yes

Keywords

Confocal laser scanning microscope
DNA
DNA complex
Gene transfer
Lipoglutamide
Poly(ethylene glycol)
Subcellular distribution

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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10.1163/156856297X00245

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title = "Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails",

abstract = "Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.",

keywords = "Confocal laser scanning microscope, DNA, DNA complex, Gene transfer, Lipoglutamide, Poly(ethylene glycol), Subcellular distribution",

author = "Toshinori Sato and Hiroyuki Akino and Yoko Shoji and Jingoro Shimada and Yoshio Okahata",

note = "Funding Information: This work was supported in part by Terumo Life Science Foundation and a Grant-in-aid for Scientific Research (No. 08266219) from The Ministry of Education, Science and Culture.",

year = "1997",

month = jan,

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doi = "10.1163/156856297X00245",

language = "English",

volume = "9",

pages = "31--42",

journal = "Journal of Biomaterials Science, Polymer Edition",

issn = "0920-5063",

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T1 - Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails

AU - Sato, Toshinori

AU - Akino, Hiroyuki

AU - Shoji, Yoko

AU - Shimada, Jingoro

AU - Okahata, Yoshio

N1 - Funding Information: This work was supported in part by Terumo Life Science Foundation and a Grant-in-aid for Scientific Research (No. 08266219) from The Ministry of Education, Science and Culture.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.

AB - Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.

KW - Confocal laser scanning microscope

KW - DNA

KW - DNA complex

KW - Gene transfer

KW - Lipoglutamide

KW - Poly(ethylene glycol)

KW - Subcellular distribution

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JF - Journal of Biomaterials Science, Polymer Edition

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Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails

Abstract

Keywords

ASJC Scopus subject areas

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