TY - JOUR
T1 - Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa
AU - Kishi, Kenji
AU - Yamazaki, Kazuya
AU - Yasuda, Ikuko
AU - Yahagi, Naohisa
AU - Ichinose, Masao
AU - Tsuchiya, Yuichi
AU - Athauda, Senarath B.P.
AU - Inoue, Hideshi
AU - Takahashi, Kenji
PY - 2001
Y1 - 2001
N2 - Previously we isolated and characterized a membrane-bound, arginine-specific serine protease from pig intestinal mucosa [J. Biol. Chem. 269, 32985-32991 (1994)]. For further characterization of this type of enzyme, we cloned a cDNA from rat intestinal mucosa encoding the precursor of a similar protease. The partial amino acid sequences determined for the pig enzyme were found to be shared almost completely by the rat enzyme. The serine protease domain of the rat enzyme, heterologously expressed in Escherichia coli, specifically cleaved Arg (or Lys)-X bonds with a marked preference for Arg-Arg or Arg-Lys, similar to the pig enzyme. The mRNA for the rat enzyme was shown to be distributed mainly in intestine, and the enzyme was detected in the duodenal mucosa as a 70 kDa protein. Immunohistochemical analysis of the small intestinal tissue showed that the enzyme is localized mainly on brushborder membranes.
AB - Previously we isolated and characterized a membrane-bound, arginine-specific serine protease from pig intestinal mucosa [J. Biol. Chem. 269, 32985-32991 (1994)]. For further characterization of this type of enzyme, we cloned a cDNA from rat intestinal mucosa encoding the precursor of a similar protease. The partial amino acid sequences determined for the pig enzyme were found to be shared almost completely by the rat enzyme. The serine protease domain of the rat enzyme, heterologously expressed in Escherichia coli, specifically cleaved Arg (or Lys)-X bonds with a marked preference for Arg-Arg or Arg-Lys, similar to the pig enzyme. The mRNA for the rat enzyme was shown to be distributed mainly in intestine, and the enzyme was detected in the duodenal mucosa as a 70 kDa protein. Immunohistochemical analysis of the small intestinal tissue showed that the enzyme is localized mainly on brushborder membranes.
KW - Brushborder membrane
KW - Intestinal mucosa
KW - Serine protease
KW - Subcellular localization
KW - Substrate specificity
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U2 - 10.1093/oxfordjournals.jbchem.a003002
DO - 10.1093/oxfordjournals.jbchem.a003002
M3 - Article
C2 - 11530019
AN - SCOPUS:0034848571
SN - 0021-924X
VL - 130
SP - 425
EP - 430
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 3
ER -