Characterization of a neutral and an acidic polysaccharide having immunological activities from the root of Paeonia lactiflora

M. Tomoda, K. Matsumoto, N. Shimizu, R. Gonda, N. Ohara

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A neutral and an acidic polysaccharide, called peonan SA and peonan SB, were isolated from the root of Paeonia lactiflora PALLAS. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 11.2 x 104 and 25.0 x 104, respectively. Peonan SA is composed of L-arabinose: D-galactose: D-glucose in the molar ratio of 1:1:28, and peonan SB is composed of L-arabinose: D-galactose: D-galacturonic acid in the molar ratio of 4:3:2, in addition to small amounts of peptide moieties. About forty percent of the hexuronic acid residues in peonan SB exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly 3,4- and 4,6-branched α-D-glucan type units (peonan SA) and principally α-1,5-linked L-arabino-β-3,6-branched D-galactan type structural units (peonan SB). Both polysaccharides, especially in peonan SB, showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test and considerable anti-complementary activity.

Original languageEnglish
Pages (from-to)1207-1210
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume16
Issue number12
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Paeonia
Arabinose
Galactose
Polysaccharides
Hexuronic Acids
Mononuclear Phagocyte System
Methylation
Gel Chromatography
Electrophoresis
Esters
Magnetic Resonance Spectroscopy
Carbon
Glucose
Peptides
galactan F
metaperiodate
galacturonic acid
polyglucosan

Keywords

  • acidic arabinogalactan
  • arabinogalacto-glucan
  • immunological activity
  • Paeonia lactiflora
  • polysaccharide structure

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Characterization of a neutral and an acidic polysaccharide having immunological activities from the root of Paeonia lactiflora. / Tomoda, M.; Matsumoto, K.; Shimizu, N.; Gonda, R.; Ohara, N.

In: Biological and Pharmaceutical Bulletin, Vol. 16, No. 12, 1993, p. 1207-1210.

Research output: Contribution to journalArticle

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AU - Gonda, R.

AU - Ohara, N.

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N2 - A neutral and an acidic polysaccharide, called peonan SA and peonan SB, were isolated from the root of Paeonia lactiflora PALLAS. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 11.2 x 104 and 25.0 x 104, respectively. Peonan SA is composed of L-arabinose: D-galactose: D-glucose in the molar ratio of 1:1:28, and peonan SB is composed of L-arabinose: D-galactose: D-galacturonic acid in the molar ratio of 4:3:2, in addition to small amounts of peptide moieties. About forty percent of the hexuronic acid residues in peonan SB exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly 3,4- and 4,6-branched α-D-glucan type units (peonan SA) and principally α-1,5-linked L-arabino-β-3,6-branched D-galactan type structural units (peonan SB). Both polysaccharides, especially in peonan SB, showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test and considerable anti-complementary activity.

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