Characterization of a novel D-Glycero-D-talo-oct-2-ulosonic acid-substituted lipid A moiety in the lipopolysaccharide produced by the acetic acid bacterium Acetobacter pasteurianus NBRC 3283

Masahito Hashimoto, Mami Ozono, Maiko Furuyashiki, Risako Baba, Shuhei Hashiguchi, Yasuo Suda, Koichi Fukase, Yukari Fujimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acetobacter pasteurianus is an aerobic Gram-negative rod that is used in the fermentation process used to produce the traditional Japanese black rice vinegar kurozu. Previously, we found that a hydrophobic fraction derived from kurozu stimulates Toll-like receptors to produce cytokines. LPSs, particularly LPS from A. pasteurianus, are strong candidates for the immunostimulatory component of kurozu. The LPS of A. pasteurianus remains stable in acidic conditions during the 2 years of the abovementioned fermentation process. Thus, we hypothesized that its stability results from its structure. In this study, we isolated the LPS produced by A. pasteurianus NBRC 3283 bacterial cells and characterized the structure of its lipid A component. The lipid A moiety was obtained by standard weak acid hydrolysis of the LPS. However, the hydrolysis was incomplete because a certain proportion of the LPS contained acid-stable D-glycero-D-talo-oct-2-ulosonic acid (Ko) residues instead of the acid-labile 3-deoxy-D-manno-oct-2-ulosonic acid residues that are normally found in typical LPS. Even so, we obtained a Ko-substituted lipid A with a novel sugar backbone, α-Man(1-4)[α-Ko(2-6)]β-GlcN3N(1-6)α-GlcN(1-1)α-GlcA. Its reducing end GlcN(1-1)GlcA bond was also found to be quite acid-stable. Six fatty acids were attached to the backbone. Both the whole LPS and the lipid A moiety induced TNF-α production in murine cells via Toll-like receptor 4, although their activity was weaker than those of Escherichia coli LPS and lipid A. These results suggest that the structurally atypical A. pasteurianus lipid A found in this study remains stable and, hence, retains its immunostimulatory activity during acetic acid fermentation.

Original languageEnglish
Pages (from-to)21184-21194
Number of pages11
JournalJournal of Biological Chemistry
Volume291
Issue number40
DOIs
Publication statusPublished - 2016 Sep 30

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Acetobacter
Lipid A
Acetic Acid
Lipopolysaccharides
Bacteria
Acids
Fermentation
Hydrolysis
Toll-Like Receptor 4
Toll-Like Receptors
Sugars
Escherichia coli
Fatty Acids
Cytokines

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of a novel D-Glycero-D-talo-oct-2-ulosonic acid-substituted lipid A moiety in the lipopolysaccharide produced by the acetic acid bacterium Acetobacter pasteurianus NBRC 3283. / Hashimoto, Masahito; Ozono, Mami; Furuyashiki, Maiko; Baba, Risako; Hashiguchi, Shuhei; Suda, Yasuo; Fukase, Koichi; Fujimoto, Yukari.

In: Journal of Biological Chemistry, Vol. 291, No. 40, 30.09.2016, p. 21184-21194.

Research output: Contribution to journalArticle

Hashimoto, Masahito ; Ozono, Mami ; Furuyashiki, Maiko ; Baba, Risako ; Hashiguchi, Shuhei ; Suda, Yasuo ; Fukase, Koichi ; Fujimoto, Yukari. / Characterization of a novel D-Glycero-D-talo-oct-2-ulosonic acid-substituted lipid A moiety in the lipopolysaccharide produced by the acetic acid bacterium Acetobacter pasteurianus NBRC 3283. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 40. pp. 21184-21194.
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AU - Ozono, Mami

AU - Furuyashiki, Maiko

AU - Baba, Risako

AU - Hashiguchi, Shuhei

AU - Suda, Yasuo

AU - Fukase, Koichi

AU - Fujimoto, Yukari

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