Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene

J. Suzuki, S. Kobayashi, M. Imada, M. E. Tolba, T. Takeuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An Entamoeba histolytica strain (BF-841 cl1) that originated from Burkina Faso, Africa presented with novel, polymorphic genotypes of the serine-rich E. histolytica protein and the anodic hexokinase-2 (HXK-2) isoenzyme band, which showed less electrophoretic mobility than that of an E. histolytica reference strain [HM-1:IMSS cl6 (zymodeme (Z)-II)] by starch gel electrophoresis and isoelectric focusing (IEF). The HXK-2 gene of BF-841 cl1 had amino acid variations at four positions compared to the sequence of HM-1: IMSS cl6. These variations were absent from the sequences of four other E. histolytica strains with different zymodemes [KU27 (Z-II), SAW1627 (Z-IIα-), SAW755CR clB (Z-XIV), and KU2 (Z-XIX)]. The results of IEF showed no difference in the substrate specificity of HXK (HXK-1 and HXK-2) between BF-841 cl1 and the three reference E. histolytica strains (HM-1:IMSS cl6, SAW755 clB, and KU27). It was also confirmed that BF-841 cl1 was able to form liver abscesses in Syrian hamsters.

Original languageEnglish
Pages (from-to)287-294
Number of pages8
JournalParasite
Volume18
Issue number4
Publication statusPublished - 2011 Nov

Fingerprint

Burkina Faso
Entamoeba histolytica
Hexokinase
hexokinase
Isoelectric Focusing
Genes
isoelectric focusing
genes
Starch Gel Electrophoresis
Liver Abscess
Mesocricetus
liver abscess
Substrate Specificity
Isoenzymes
starch gels
substrate specificity
serine
Genotype
gel electrophoresis
isozymes

Keywords

  • Entamoeba histolytica
  • Genetic variation
  • Hexokinase
  • Isoelectric focusing
  • Isoenzyme

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology

Cite this

Suzuki, J., Kobayashi, S., Imada, M., Tolba, M. E., & Takeuchi, T. (2011). Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene. Parasite, 18(4), 287-294.

Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene. / Suzuki, J.; Kobayashi, S.; Imada, M.; Tolba, M. E.; Takeuchi, T.

In: Parasite, Vol. 18, No. 4, 11.2011, p. 287-294.

Research output: Contribution to journalArticle

Suzuki, J, Kobayashi, S, Imada, M, Tolba, ME & Takeuchi, T 2011, 'Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene', Parasite, vol. 18, no. 4, pp. 287-294.
Suzuki, J. ; Kobayashi, S. ; Imada, M. ; Tolba, M. E. ; Takeuchi, T. / Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene. In: Parasite. 2011 ; Vol. 18, No. 4. pp. 287-294.
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