Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

Original languageEnglish
Pages (from-to)3473-3480
Number of pages8
JournalFEBS Letters
Volume579
Issue number17
DOIs
Publication statusPublished - 2005 Jul 27

Fingerprint

Fetal Stem Cells
ATP-Binding Cassette Transporters
Neural Stem Cells
Stem cells
Stem Cells
Rhodamine 123
Nestin
Cell membranes
Fractionation
Microsomes
Gene expression
Cell Differentiation
Adenosine Triphosphate
Immunohistochemistry
Cell Membrane
Gene Expression
Polymerase Chain Reaction

Keywords

  • ABC transporter
  • ABCB1
  • Cell surface marker
  • Neural stem cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Islam, M. O., Kanemura, Y., Tajria, J., Mori, H., Kobayashi, S., Shofuda, T., ... Okano, H. (2005). Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. FEBS Letters, 579(17), 3473-3480. https://doi.org/10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. / Islam, Mohammed Omedul; Kanemura, Yonehiro; Tajria, Jesmin; Mori, Hideki; Kobayashi, Satoshi; Shofuda, Tomoko; Miyake, Jun; Hara, Masayuki; Yamasaki, Mami; Okano, Hideyuki.

In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3473-3480.

Research output: Contribution to journalArticle

Islam, MO, Kanemura, Y, Tajria, J, Mori, H, Kobayashi, S, Shofuda, T, Miyake, J, Hara, M, Yamasaki, M & Okano, H 2005, 'Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells', FEBS Letters, vol. 579, no. 17, pp. 3473-3480. https://doi.org/10.1016/j.febslet.2005.05.019
Islam MO, Kanemura Y, Tajria J, Mori H, Kobayashi S, Shofuda T et al. Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. FEBS Letters. 2005 Jul 27;579(17):3473-3480. https://doi.org/10.1016/j.febslet.2005.05.019
Islam, Mohammed Omedul ; Kanemura, Yonehiro ; Tajria, Jesmin ; Mori, Hideki ; Kobayashi, Satoshi ; Shofuda, Tomoko ; Miyake, Jun ; Hara, Masayuki ; Yamasaki, Mami ; Okano, Hideyuki. / Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. In: FEBS Letters. 2005 ; Vol. 579, No. 17. pp. 3473-3480.
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