Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam; Yonehiro Kanemura; Jesmin Tajria; Hideki Mori; Satoshi Kobayashi; Tomoko Shofuda; Jun Miyake; Masayuki Hara; Mami Yamasaki; Hideyuki Okano

doi:10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano

Department of Physiology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

Original language	English
Pages (from-to)	3473-3480
Number of pages	8
Journal	FEBS Letters
Volume	579
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2005.05.019
Publication status	Published - 2005 Jul 27

Keywords

ABC transporter
ABCB1
Cell surface marker
Neural stem cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2005.05.019

Fingerprint Dive into the research topics of 'Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells'. Together they form a unique fingerprint.

Cite this

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. / Islam, Mohammed Omedul; Kanemura, Yonehiro; Tajria, Jesmin; Mori, Hideki; Kobayashi, Satoshi; Shofuda, Tomoko; Miyake, Jun; Hara, Masayuki; Yamasaki, Mami; Okano, Hideyuki.

In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3473-3480.

Research output: Contribution to journal › Article › peer-review

@article{a491d50d66d74c5f91f96d6362b78b46,

title = "Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells",

abstract = "We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.",

keywords = "ABC transporter, ABCB1, Cell surface marker, Neural stem cell",

author = "Islam, {Mohammed Omedul} and Yonehiro Kanemura and Jesmin Tajria and Hideki Mori and Satoshi Kobayashi and Tomoko Shofuda and Jun Miyake and Masayuki Hara and Mami Yamasaki and Hideyuki Okano",

note = "Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2005",

month = jul,

day = "27",

doi = "10.1016/j.febslet.2005.05.019",

language = "English",

volume = "579",

pages = "3473--3480",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "17",

}

TY - JOUR

T1 - Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

AU - Islam, Mohammed Omedul

AU - Kanemura, Yonehiro

AU - Tajria, Jesmin

AU - Mori, Hideki

AU - Kobayashi, Satoshi

AU - Shofuda, Tomoko

AU - Miyake, Jun

AU - Hara, Masayuki

AU - Yamasaki, Mami

AU - Okano, Hideyuki

N1 - Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2005/7/27

Y1 - 2005/7/27

N2 - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

AB - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

KW - ABC transporter

KW - ABCB1

KW - Cell surface marker

KW - Neural stem cell

UR - http://www.scopus.com/inward/record.url?scp=21244452807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244452807&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2005.05.019

DO - 10.1016/j.febslet.2005.05.019

M3 - Article

C2 - 15950972

AN - SCOPUS:21244452807

VL - 579

SP - 3473

EP - 3480

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 17

ER -

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this