Abstract
We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
| Original language | English |
|---|---|
| Pages (from-to) | 3473-3480 |
| Number of pages | 8 |
| Journal | FEBS Letters |
| Volume | 579 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2005 Jul 27 |
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Keywords
- ABC transporter
- ABCB1
- Cell surface marker
- Neural stem cell
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. / Islam, Mohammed Omedul; Kanemura, Yonehiro; Tajria, Jesmin; Mori, Hideki; Kobayashi, Satoshi; Shofuda, Tomoko; Miyake, Jun; Hara, Masayuki; Yamasaki, Mami; Okano, Hideyuki.
In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3473-3480.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells
AU - Islam, Mohammed Omedul
AU - Kanemura, Yonehiro
AU - Tajria, Jesmin
AU - Mori, Hideki
AU - Kobayashi, Satoshi
AU - Shofuda, Tomoko
AU - Miyake, Jun
AU - Hara, Masayuki
AU - Yamasaki, Mami
AU - Okano, Hideyuki
PY - 2005/7/27
Y1 - 2005/7/27
N2 - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
AB - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
KW - ABC transporter
KW - ABCB1
KW - Cell surface marker
KW - Neural stem cell
UR - http://www.scopus.com/inward/record.url?scp=21244452807&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21244452807&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2005.05.019
DO - 10.1016/j.febslet.2005.05.019
M3 - Article
VL - 579
SP - 3473
EP - 3480
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 17
ER -