Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

Original languageEnglish
Pages (from-to)3473-3480
Number of pages8
JournalFEBS Letters
Volume579
Issue number17
DOIs
Publication statusPublished - 2005 Jul 27

Keywords

  • ABC transporter
  • ABCB1
  • Cell surface marker
  • Neural stem cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells'. Together they form a unique fingerprint.

  • Cite this

    Islam, M. O., Kanemura, Y., Tajria, J., Mori, H., Kobayashi, S., Shofuda, T., Miyake, J., Hara, M., Yamasaki, M., & Okano, H. (2005). Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. FEBS Letters, 579(17), 3473-3480. https://doi.org/10.1016/j.febslet.2005.05.019