Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam; Yonehiro Kanemura; Jesmin Tajria; Hideki Mori; Satoshi Kobayashi; Tomoko Shofuda; Jun Miyake; Masayuki Hara; Mami Yamasaki; Hideyuki Okano

doi:10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano

Department of Physiology

Research output: Contribution to journal › Article

37 Citations (Scopus)

Abstract

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

Original language	English
Pages (from-to)	3473-3480
Number of pages	8
Journal	FEBS Letters
Volume	579
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2005.05.019
Publication status	Published - 2005 Jul 27

Keywords

ABC transporter
ABCB1
Cell surface marker
Neural stem cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2005.05.019

Fingerprint Dive into the research topics of 'Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells'. Together they form a unique fingerprint.

Cite this

Islam, M. O., Kanemura, Y., Tajria, J., Mori, H., Kobayashi, S., Shofuda, T., Miyake, J., Hara, M., Yamasaki, M., & Okano, H. (2005). Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. FEBS Letters, 579(17), 3473-3480. https://doi.org/10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. / Islam, Mohammed Omedul; Kanemura, Yonehiro; Tajria, Jesmin; Mori, Hideki; Kobayashi, Satoshi; Shofuda, Tomoko; Miyake, Jun; Hara, Masayuki; Yamasaki, Mami; Okano, Hideyuki.

In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3473-3480.

Research output: Contribution to journal › Article

@article{a491d50d66d74c5f91f96d6362b78b46,

title = "Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells",

abstract = "We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.",

keywords = "ABC transporter, ABCB1, Cell surface marker, Neural stem cell",

author = "Islam, {Mohammed Omedul} and Yonehiro Kanemura and Jesmin Tajria and Hideki Mori and Satoshi Kobayashi and Tomoko Shofuda and Jun Miyake and Masayuki Hara and Mami Yamasaki and Hideyuki Okano",

year = "2005",

month = jul,

day = "27",

doi = "10.1016/j.febslet.2005.05.019",

language = "English",

volume = "579",

pages = "3473--3480",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "17",

}

TY - JOUR

T1 - Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

AU - Islam, Mohammed Omedul

AU - Kanemura, Yonehiro

AU - Tajria, Jesmin

AU - Mori, Hideki

AU - Kobayashi, Satoshi

AU - Shofuda, Tomoko

AU - Miyake, Jun

AU - Hara, Masayuki

AU - Yamasaki, Mami

AU - Okano, Hideyuki

PY - 2005/7/27

Y1 - 2005/7/27

N2 - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

AB - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

KW - ABC transporter

KW - ABCB1

KW - Cell surface marker

KW - Neural stem cell

UR - http://www.scopus.com/inward/record.url?scp=21244452807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244452807&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2005.05.019

DO - 10.1016/j.febslet.2005.05.019

M3 - Article

C2 - 15950972

AN - SCOPUS:21244452807

VL - 579

SP - 3473

EP - 3480

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 17

ER -