Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam; Yonehiro Kanemura; Jesmin Tajria; Hideki Mori; Satoshi Kobayashi; Tomoko Shofuda; Jun Miyake; Masayuki Hara; Mami Yamasaki; Hideyuki Okano

doi:10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano

Department of Physiology

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

Original language	English
Pages (from-to)	3473-3480
Number of pages	8
Journal	FEBS Letters
Volume	579
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2005.05.019
Publication status	Published - 2005 Jul 27

Keywords

ABC transporter
ABCB1
Cell surface marker
Neural stem cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2005.05.019

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Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells. / Islam, Mohammed Omedul; Kanemura, Yonehiro; Tajria, Jesmin; Mori, Hideki; Kobayashi, Satoshi; Shofuda, Tomoko; Miyake, Jun; Hara, Masayuki; Yamasaki, Mami; Okano, Hideyuki.

In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3473-3480.

Research output: Contribution to journal › Article › peer-review

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abstract = "We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.",

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note = "Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

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AU - Hara, Masayuki

AU - Yamasaki, Mami

AU - Okano, Hideyuki

N1 - Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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N2 - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

AB - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

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Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Abstract

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