Characterization of an acidic polysaccharide with immunological activities from the tuber of Pinellia ternata

Ryoko Gonda, M. Tomoda, N. Shimizu, N. Ohara, H. Takagi, S. Hoshino

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An acidic polysaccharide, called pinellian PA, was isolated from the tuber of Pinellia ternata BREIT. It was homogeneous on electrophoresis and gel chromatography, and its molecular mass was estimated to be 11.8 x 104. Pinellian PA is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid: D-glucuronic acid in the molar ratio of 5:15:1:3:3, in addition to small amounts of O-acetyl groups and peptide moieties. Reduction of carboxyl groups, methylation-analysis and nuclear magnetic resonance studies show that the core structural features include a backbone chain composed of β-1,3-linked D-galactose units. Some of the galactose-units in the backbone carry β-1,6-linked D-galactosyl side-chains at position 6. Pinellian PA produces significant potentiation of the reticuloendothelial system, as shown by a carbon clearance test, and also exhibits potent anti-complementary activity.

Original languageEnglish
Pages (from-to)1549-1553
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume17
Issue number12
Publication statusPublished - 1994

Fingerprint

Pinellia
Galactose
Polysaccharides
Rhamnose
Glucuronic Acid
Mononuclear Phagocyte System
Arabinose
Methylation
Gel Chromatography
Electrophoresis
Magnetic Resonance Spectroscopy
Carbon
Peptides

Keywords

  • Acidic arabinogalactan
  • Immunological activity
  • Pinellia ternata
  • Pinellian PA
  • Polysaccharide structure
  • Tuber

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Characterization of an acidic polysaccharide with immunological activities from the tuber of Pinellia ternata. / Gonda, Ryoko; Tomoda, M.; Shimizu, N.; Ohara, N.; Takagi, H.; Hoshino, S.

In: Biological and Pharmaceutical Bulletin, Vol. 17, No. 12, 1994, p. 1549-1553.

Research output: Contribution to journalArticle

Gonda, Ryoko ; Tomoda, M. ; Shimizu, N. ; Ohara, N. ; Takagi, H. ; Hoshino, S. / Characterization of an acidic polysaccharide with immunological activities from the tuber of Pinellia ternata. In: Biological and Pharmaceutical Bulletin. 1994 ; Vol. 17, No. 12. pp. 1549-1553.
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