Characterization of angiotensin II receptor type 2 during differentiation and apoptosis of rat ovarian cultured granulosa cells

Masami Tanaka, Junji Ohnishi, Yasuhiro Ozawa, Masaiaka Sugimoto, Satoshi Usuki, Mitsuhide Naruse, Kazuo Murakami, Hitoshi Miyazaki

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We examined the change in the content of angiotensin II (AII) receptor type 2 (AT2) during differentiation and apoptosis of rat ovarian granulosa cells in culture. The AT2 content was not changed by follicle stimulating hormone (FSH), a differentiation factor of granulosa cells, but was markedly increased in FSH-free media. The cells cultured without FSH underwent internucleosomal DNA fragmentation characteristic of apoptosis, which occurs during follicle atresia. AII augmented the increase in the AT2 content in the absence of FSH. This AII-induced augmentation was suppressed by the AT2-selective antagonist PD123319 but not by Dup753, an antagonist specific for type 1 receptor, suggesting that AII up-regulates the AT2 expression via AT2 itself. These data strongly support the hypothesis that AT2 might modulate the onset and progression of follicle atresia involving apoptosis of granulosa cells.

Original languageEnglish
Pages (from-to)593-598
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume207
Issue number2
DOIs
Publication statusPublished - 1995 Feb 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization of angiotensin II receptor type 2 during differentiation and apoptosis of rat ovarian cultured granulosa cells'. Together they form a unique fingerprint.

  • Cite this