Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy

A. Baba, T. Yoshikawa, M. Chino, A. Murayama, K. Mitani, S. Nakagawa, I. Fujii, M. Shimada, M. Akaishi, S. Iwanaga, Y. Asakura, K. Fukuda, H. Mitamura, S. Ogawa

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Abstract

Few previous reports have comprehensively screened all the anti-myocardial autoantibodies (AMCA) in relation to other clinical profiles in patients with idiopathic dilated cardiomyopathy (IDC), so the present study used both immunohistochemistry (FITC) and immunoblotting (IB) for screening patients with IDC in order to characterize the clinical significance of AMCA. Sera were collected from 100 patients with IDC and age-matched 100 healthy control subjects (CTL). For FITC, an unfixed frozen section of human myocardium was used for the standard indirect immunofluorescence; for IB, total cardiac homogenates of the same myocardium were blotted to serum at 2 sets of dilution (1:200 and 1:10,000). The positive rates of AMCA detection for each method were as follows (IDC vs CTL); 39% vs 6% for FITC, 38% vs 4% for IB (1:200), and 10% vs 0% for IB (1:10,000). Fifty-nine patients with IDC and 8 CTL were positive for AMCA by either method, and 18 patients with IDC and 2 CTL were positive for AMCA by both methods. IB-positivity at 1:200 was an independent predictor by multiple logistic regression analysis of non-sustained ventricular tachycardias as well as left ventricular end-diastolic diameter and plasma norepinephrine concentration.

Original languageEnglish
Pages (from-to)867-873
Number of pages7
JournalJAPANESE CIRCULATION JOURNAL
Volume65
Issue number10
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

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Keywords

  • Autoantibodies
  • Dilated cardiomyopathy
  • Immunoblotting
  • Immunohistochemistry

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Baba, A., Yoshikawa, T., Chino, M., Murayama, A., Mitani, K., Nakagawa, S., Fujii, I., Shimada, M., Akaishi, M., Iwanaga, S., Asakura, Y., Fukuda, K., Mitamura, H., & Ogawa, S. (2001). Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy. JAPANESE CIRCULATION JOURNAL, 65(10), 867-873. https://doi.org/10.1253/jcj.65.867