Characterization of AOC2 gene encoding a copper-binding amine oxidase expressed specifically in retina

Qiang Zhang, Yukihiko Mashima, Setsuko Noda, Yutaka Imamura, Jun Kudo, Nobuyoshi Shimizu, Takatsune Nishiyama, Shinsuke Umeda, Yoshihisa Oguchi, Yasuhiko Tanaka, Takeshi Iwata

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have previously cloned a human, retina-specific, amine oxidase gene (RAO, gene symbol: AOC2), a member of the copper-binding amine oxidase super family. AOC2 shares sequence identity with the human kidney amine oxidase gene (KAO, gene symbol: AOC1) and the vascular adhesion protein-1 gene (VAP-1, gene symbol: AOC3). For further analysis of AOC2, the sequences surrounding the human AOC2 and the complete mouse and partial rat homologue of AOC2 were cloned for characterization. Real-time quantitative PCR, in situ hybridization, and immunohistochemistry were performed to determine the specific expression of AOC2 in the mouse retina and especially in the retinal ganglion cells. Our results demonstrated that the copper-binding motif and the enzyme active site of AOC1 and AOC3 were both conserved in mouse AOC2. The human and mouse AOC2 was flanked by two genes, the Psme3 gene for PA-28 gamma subunit and, surprisingly, the AOC3 gene. Rat AOC2 contained a stop codon that terminated the peptide length to 127 amino acids. The presence of human and rat AOC pseudogene in this region, in addition to the tandemly positioned two AOC genes, indicates the possibility of successful AOC3 replication to retina-specific AOC2 for human and mouse but unsuccessful for rat.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalGene
Volume318
Issue number1-2
DOIs
Publication statusPublished - 2003 Oct 30

Fingerprint

Amine Oxidase (Copper-Containing)
Retina
Genes
Amines
Oxidoreductases
Pseudogenes
Retinal Ganglion Cells
Terminator Codon
In Situ Hybridization
Sequence Analysis
Blood Vessels
Real-Time Polymerase Chain Reaction
Copper
Catalytic Domain
Immunohistochemistry
Kidney
Amino Acids
Peptides

Keywords

  • Amines
  • Copper amine oxidase
  • GABA
  • Oxidase
  • Retina
  • Retinal ganglion cells

ASJC Scopus subject areas

  • Genetics

Cite this

Zhang, Q., Mashima, Y., Noda, S., Imamura, Y., Kudo, J., Shimizu, N., ... Iwata, T. (2003). Characterization of AOC2 gene encoding a copper-binding amine oxidase expressed specifically in retina. Gene, 318(1-2), 45-53. https://doi.org/10.1016/S0378-1119(03)00753-4

Characterization of AOC2 gene encoding a copper-binding amine oxidase expressed specifically in retina. / Zhang, Qiang; Mashima, Yukihiko; Noda, Setsuko; Imamura, Yutaka; Kudo, Jun; Shimizu, Nobuyoshi; Nishiyama, Takatsune; Umeda, Shinsuke; Oguchi, Yoshihisa; Tanaka, Yasuhiko; Iwata, Takeshi.

In: Gene, Vol. 318, No. 1-2, 30.10.2003, p. 45-53.

Research output: Contribution to journalArticle

Zhang, Q, Mashima, Y, Noda, S, Imamura, Y, Kudo, J, Shimizu, N, Nishiyama, T, Umeda, S, Oguchi, Y, Tanaka, Y & Iwata, T 2003, 'Characterization of AOC2 gene encoding a copper-binding amine oxidase expressed specifically in retina', Gene, vol. 318, no. 1-2, pp. 45-53. https://doi.org/10.1016/S0378-1119(03)00753-4
Zhang, Qiang ; Mashima, Yukihiko ; Noda, Setsuko ; Imamura, Yutaka ; Kudo, Jun ; Shimizu, Nobuyoshi ; Nishiyama, Takatsune ; Umeda, Shinsuke ; Oguchi, Yoshihisa ; Tanaka, Yasuhiko ; Iwata, Takeshi. / Characterization of AOC2 gene encoding a copper-binding amine oxidase expressed specifically in retina. In: Gene. 2003 ; Vol. 318, No. 1-2. pp. 45-53.
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abstract = "We have previously cloned a human, retina-specific, amine oxidase gene (RAO, gene symbol: AOC2), a member of the copper-binding amine oxidase super family. AOC2 shares sequence identity with the human kidney amine oxidase gene (KAO, gene symbol: AOC1) and the vascular adhesion protein-1 gene (VAP-1, gene symbol: AOC3). For further analysis of AOC2, the sequences surrounding the human AOC2 and the complete mouse and partial rat homologue of AOC2 were cloned for characterization. Real-time quantitative PCR, in situ hybridization, and immunohistochemistry were performed to determine the specific expression of AOC2 in the mouse retina and especially in the retinal ganglion cells. Our results demonstrated that the copper-binding motif and the enzyme active site of AOC1 and AOC3 were both conserved in mouse AOC2. The human and mouse AOC2 was flanked by two genes, the Psme3 gene for PA-28 gamma subunit and, surprisingly, the AOC3 gene. Rat AOC2 contained a stop codon that terminated the peptide length to 127 amino acids. The presence of human and rat AOC pseudogene in this region, in addition to the tandemly positioned two AOC genes, indicates the possibility of successful AOC3 replication to retina-specific AOC2 for human and mouse but unsuccessful for rat.",
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AU - Nishiyama, Takatsune

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