Characterization of bullous pemphigoid antibodies by use of recombinant bullous pemphigoid antigen proteins

Masaru Tanaka, Takashi Hashimoto, Masayuki Amagai, Nobuyoshi Shimizu, Naoko Ikeguchi, Takuichi Tsubata, Akira Hasegawa, Keizaburo Miki, Takeji Nishikawa

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47 Citations (Scopus)

Abstract

The seroreactivity of patients with bullous pemphigoid (BP) to recombinant proteins representing sequences in the carboxyl domain of the murine 230-kD BP antigen (BPA) was determined. Sera from 133 patients with BP, 20 patients with pemphigus, and 21 normal subjects were examined by Western blotting by using two recombinant proteins: RP120 (MW = 120 kD), representing the C-terminal half of the 230-kD BPA, and RP60 (MW = 60 kD), representing the C-terminal quarter. These RP120 and RP60 were recognized by 84% and 61%, respectively, of the BP sera that reacted with the 230-kD BPA in epidermal extract, and not by any of pemphigus or normal sera. Furthermore, these RP120 and RP60 were not recognized by any BP sera that reacted only with the 170-kD BPA, which is known to be another major BPA. These findings indicate that one or more of the major antigenic regions localizes in the carboxyl-half domain of the 230-kD BPA, and also suggest that the 230-kD BPA may be distinct from the 170-kD BPA.

Original languageEnglish
Pages (from-to)725-728
Number of pages4
JournalJournal of Investigative Dermatology
Volume97
Issue number4
DOIs
Publication statusPublished - 1991 Oct

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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  • Cite this

    Tanaka, M., Hashimoto, T., Amagai, M., Shimizu, N., Ikeguchi, N., Tsubata, T., Hasegawa, A., Miki, K., & Nishikawa, T. (1991). Characterization of bullous pemphigoid antibodies by use of recombinant bullous pemphigoid antigen proteins. Journal of Investigative Dermatology, 97(4), 725-728. https://doi.org/10.1111/1523-1747.ep12484223