Characterization of cDNA encoding full-length mouse platelet glycoprotein IX

T. Kitaguchi, Mitsuru Murata, H. Ambo, Y. Ikeda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glycoprotein (GP) IX is a platelet membrane 20 kD protein, which is associated with GPIba, GPIbβ, and GPV to form GPIb/IX/V complex. GPIb/IX/V complex is a major receptor for yon Willebrand factor, which mediates platelet adhesion and aggregation under high shear stress conditions. The relevance of this receptor for hemostasis has been implicated by a congenital bleeding disorder lacking the receptor, Bernard-Soulier syndrome. All subunits for the human receptor have been cloned and characterized. However, the function of GPIX is still elusive. To obtain further information of GPIX, we have determined a cDNA sequence of mouse GPIX (811 bp). The deduced amino- acid sequence (177aa) was 71% identical to the human GPIX protein. All cysteine residues in extracytoplasmic domain and putative N-linked glycosylation site (Asn44) were conserved. Mouse GPIX contained a leucine- rich glycoprotein sequence composed of 24 amino acids, as did human GPIX.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalBlood Coagulation and Fibrinolysis
Volume9
Issue number4
Publication statusPublished - 1998

Fingerprint

Platelet Glycoprotein GPIb-IX Complex
Glycoproteins
Bernard-Soulier Syndrome
Complementary DNA
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Hemostasis
Glycosylation
Platelet Aggregation
Leucine
Cysteine
Amino Acid Sequence
Blood Platelets
Hemorrhage
Amino Acids
Membranes
Proteins
oligomycin sensitivity-conferring protein
adhesion receptor

Keywords

  • CDNA
  • Glycoprotein IX
  • Leucine-rich glycoprotein
  • Mouse
  • Platelets
  • Receptor
  • Von Willebrand factor

ASJC Scopus subject areas

  • Hematology

Cite this

Characterization of cDNA encoding full-length mouse platelet glycoprotein IX. / Kitaguchi, T.; Murata, Mitsuru; Ambo, H.; Ikeda, Y.

In: Blood Coagulation and Fibrinolysis, Vol. 9, No. 4, 1998, p. 381-385.

Research output: Contribution to journalArticle

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