Abstract
Purpose. The host response to allergens appears to be regulated by specific patterns of local cytokine production. More than 20,000 conjunctival superficial cells were collected with a special brush, a smaller version of the Cytobrush used in cervical cytology, from the upper palpebral conjunctiva. Methods. Samples were obtained by cytology brush from seven patients with allergic conjunctivitis and from seven healthy volunteers. Giemsa staining, immunocytochemistry, and flow cytometric analysis were performed. Cytokine gene expression was assayed by the reverse- transcription-polymerase chain reaction method. Results. Giemsa staining of cytocentrifuged preparations from patients with allergic conjunctivitis showed conjunctival epithelial cells with lymphocytes, mast cells, and eosinophils. In an immunohistochemically study, a few CD3- and CD4- bearing cells, but not CD20- and CD14- bearing cells, were seen in patients. In 82.6 ± 17% of the samples obtained from allergic patients, HLA-DR was present, but it was present in only 34.2 ± 17.8% of samples from control subjects (P = 0.0001) using flow cytometric analysis. Steady state transcripts of mRNA for cytokines were analyzed with RT-PCR in conjunctival cell samples, and results showed that samples from allergic conjunctivitis expressed increased transcripts of interleukin 4 and interleukin 13 but virtually no interleukin 2 or interferon-γ; six samples from seven healthy subjects expressed no interleukin 2, interleukin 4, interleukin 13, or interferon-γ transcripts. Conclusions. These results suggest that the clinical features of allergic conjunctivitis in humans are associated with a specific local pattern of proinflammatory cytokine expression.
| Original language | English |
|---|---|
| Pages (from-to) | 1350-1357 |
| Number of pages | 8 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 38 |
| Issue number | 7 |
| Publication status | Published - 1997 |
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Keywords
- Allergic conjunctivitis
- Conjunctival cells
- Cytokine
- Reverse- transcription-polymerase chain reaction
- Th2 cells
ASJC Scopus subject areas
- Ophthalmology
Cite this
Characterization of cytokine mRNA transcripts in conjunctival cells in patients with allergic conjunctivitis. / Fujishima, Hiroshi; Saito, Ichiro; Takeuchi, Tsutomu; Shinozaki, Naoshi; Tsubota, Kazuo.
In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 7, 1997, p. 1350-1357.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of cytokine mRNA transcripts in conjunctival cells in patients with allergic conjunctivitis
AU - Fujishima, Hiroshi
AU - Saito, Ichiro
AU - Takeuchi, Tsutomu
AU - Shinozaki, Naoshi
AU - Tsubota, Kazuo
PY - 1997
Y1 - 1997
N2 - Purpose. The host response to allergens appears to be regulated by specific patterns of local cytokine production. More than 20,000 conjunctival superficial cells were collected with a special brush, a smaller version of the Cytobrush used in cervical cytology, from the upper palpebral conjunctiva. Methods. Samples were obtained by cytology brush from seven patients with allergic conjunctivitis and from seven healthy volunteers. Giemsa staining, immunocytochemistry, and flow cytometric analysis were performed. Cytokine gene expression was assayed by the reverse- transcription-polymerase chain reaction method. Results. Giemsa staining of cytocentrifuged preparations from patients with allergic conjunctivitis showed conjunctival epithelial cells with lymphocytes, mast cells, and eosinophils. In an immunohistochemically study, a few CD3- and CD4- bearing cells, but not CD20- and CD14- bearing cells, were seen in patients. In 82.6 ± 17% of the samples obtained from allergic patients, HLA-DR was present, but it was present in only 34.2 ± 17.8% of samples from control subjects (P = 0.0001) using flow cytometric analysis. Steady state transcripts of mRNA for cytokines were analyzed with RT-PCR in conjunctival cell samples, and results showed that samples from allergic conjunctivitis expressed increased transcripts of interleukin 4 and interleukin 13 but virtually no interleukin 2 or interferon-γ; six samples from seven healthy subjects expressed no interleukin 2, interleukin 4, interleukin 13, or interferon-γ transcripts. Conclusions. These results suggest that the clinical features of allergic conjunctivitis in humans are associated with a specific local pattern of proinflammatory cytokine expression.
AB - Purpose. The host response to allergens appears to be regulated by specific patterns of local cytokine production. More than 20,000 conjunctival superficial cells were collected with a special brush, a smaller version of the Cytobrush used in cervical cytology, from the upper palpebral conjunctiva. Methods. Samples were obtained by cytology brush from seven patients with allergic conjunctivitis and from seven healthy volunteers. Giemsa staining, immunocytochemistry, and flow cytometric analysis were performed. Cytokine gene expression was assayed by the reverse- transcription-polymerase chain reaction method. Results. Giemsa staining of cytocentrifuged preparations from patients with allergic conjunctivitis showed conjunctival epithelial cells with lymphocytes, mast cells, and eosinophils. In an immunohistochemically study, a few CD3- and CD4- bearing cells, but not CD20- and CD14- bearing cells, were seen in patients. In 82.6 ± 17% of the samples obtained from allergic patients, HLA-DR was present, but it was present in only 34.2 ± 17.8% of samples from control subjects (P = 0.0001) using flow cytometric analysis. Steady state transcripts of mRNA for cytokines were analyzed with RT-PCR in conjunctival cell samples, and results showed that samples from allergic conjunctivitis expressed increased transcripts of interleukin 4 and interleukin 13 but virtually no interleukin 2 or interferon-γ; six samples from seven healthy subjects expressed no interleukin 2, interleukin 4, interleukin 13, or interferon-γ transcripts. Conclusions. These results suggest that the clinical features of allergic conjunctivitis in humans are associated with a specific local pattern of proinflammatory cytokine expression.
KW - Allergic conjunctivitis
KW - Conjunctival cells
KW - Cytokine
KW - Reverse- transcription-polymerase chain reaction
KW - Th2 cells
UR - http://www.scopus.com/inward/record.url?scp=0030954590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030954590&partnerID=8YFLogxK
M3 - Article
VL - 38
SP - 1350
EP - 1357
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 7
ER -