Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing.

Mikiko C. Siomi, Haruhiko Siomi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.

Original languageEnglish
Pages (from-to)59-60
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number52
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

RNA Interference
MicroRNAs
RNA
Epitopes
Jurkat Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing. / Siomi, Mikiko C.; Siomi, Haruhiko.

In: Nucleic acids symposium series (2004), No. 52, 2008, p. 59-60.

Research output: Contribution to journalArticle

@article{55cc6acd210c4e73aa3e1f24e45b858e,
title = "Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing.",
abstract = "Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.",
author = "Siomi, {Mikiko C.} and Haruhiko Siomi",
year = "2008",
language = "English",
pages = "59--60",
journal = "Nucleic acids symposium series (2004)",
issn = "1746-8272",
publisher = "Oxford University Press",
number = "52",

}

TY - JOUR

T1 - Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing.

AU - Siomi, Mikiko C.

AU - Siomi, Haruhiko

PY - 2008

Y1 - 2008

N2 - Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.

AB - Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.

UR - http://www.scopus.com/inward/record.url?scp=78649431615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649431615&partnerID=8YFLogxK

M3 - Article

SP - 59

EP - 60

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 52

ER -