Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster

Atsushi Tominaga, Masafumi Matsui, Natsuhiko Yoshikawa, Azusa Hayano, Miho Inoue-Murayama

Research output: Contribution to journalLetter

2 Citations (Scopus)

Abstract

The Japanese fire-bellied newt Cynops pyrrhogaster, endemic to mainland Japan is ranked as Near Threatened in the red list of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed fifteen novel microsatellite markers for this species using expressed sequence tag and next-generation sequencing data. The number of alleles and expected heterozygosity at each locus were 3-28 (mean = 11.6) and 0-0.97. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of C. pyrrhogaster.

Original languageEnglish
Pages (from-to)405-407
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Salamandridae
Genetic Structures
salamanders and newts
Microsatellite Repeats
Japan
microsatellite repeats
genetic variation
genetic structure
Expressed Sequence Tags
expressed sequence tags
heterozygosity
Red List
planning
Alleles
conservation management
alleles
loci
allele
marker
genetic diversity

Keywords

  • Conservation
  • Cynops pyrrhogaster
  • Expressed sequence tag
  • Genetic diversity
  • Microsatellite
  • Next-generation sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster. / Tominaga, Atsushi; Matsui, Masafumi; Yoshikawa, Natsuhiko; Hayano, Azusa; Inoue-Murayama, Miho.

In: Conservation Genetics Resources, Vol. 6, No. 2, 01.01.2014, p. 405-407.

Research output: Contribution to journalLetter

Tominaga, Atsushi ; Matsui, Masafumi ; Yoshikawa, Natsuhiko ; Hayano, Azusa ; Inoue-Murayama, Miho. / Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster. In: Conservation Genetics Resources. 2014 ; Vol. 6, No. 2. pp. 405-407.
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