Characterization of Intra/Extracellular Water States Probed by Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopic Imaging

Mutsuo Nuriya, Hiroaki Yoneyama, Kyosuke Takahashi, Philippe Leproux, Vincent Couderc, Masato Yasui, Hideaki Kano

Research output: Contribution to journalArticle

Abstract

Detailed knowledge of the water status in living organisms is crucial for understanding their physiology and pathophysiology. Here, we developed a technique to spectroscopically image water at high resolution using ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microscopy equipped with a supercontinuum light source. This system allows for the visualization of a wide spectrum of CARS signals from the fingerprint to the end of O-H stretching at a spectral resolution of ∼10 cm -1 . Application of the system to living mammalian cells revealed a spectral red shift of the O-H stretching vibrational band inside compared to outside the cells, suggesting the existence of stronger hydrogen bonds inside the cells. Furthermore, potential changes in spectra were examined by adding mannitol to the extracellular solution, which increases the osmolality outside the cells and thereby induces dehydration of the cells. Under this treatment, the red shift of the O-H stretching band was further enhanced, revealing the effects of mannitol on water states inside the cells. The methodology developed here should serve as a powerful tool for the chemical imaging of water in living cells in various biological and medical contexts.

Original languageEnglish
Pages (from-to)3928-3934
Number of pages7
JournalJournal of Physical Chemistry A
Volume123
Issue number17
DOIs
Publication statusPublished - 2019 May 2

Fingerprint

Raman scattering
Raman spectra
Stretching
Imaging techniques
Water
mannitol
Mannitol
cells
water
red shift
Cells
Spectral resolution
Physiology
Dehydration
physiology
Light sources
Hydrogen bonds
Microscopic examination
organisms
Visualization

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Characterization of Intra/Extracellular Water States Probed by Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopic Imaging. / Nuriya, Mutsuo; Yoneyama, Hiroaki; Takahashi, Kyosuke; Leproux, Philippe; Couderc, Vincent; Yasui, Masato; Kano, Hideaki.

In: Journal of Physical Chemistry A, Vol. 123, No. 17, 02.05.2019, p. 3928-3934.

Research output: Contribution to journalArticle

Nuriya, Mutsuo ; Yoneyama, Hiroaki ; Takahashi, Kyosuke ; Leproux, Philippe ; Couderc, Vincent ; Yasui, Masato ; Kano, Hideaki. / Characterization of Intra/Extracellular Water States Probed by Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopic Imaging. In: Journal of Physical Chemistry A. 2019 ; Vol. 123, No. 17. pp. 3928-3934.
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