Characterization of lipid profiles after dietary intake of polyunsaturated fatty acids using integrated untargeted and targeted lipidomics

Satoko Naoe, Hiroshi Tsugawa, Mikiko Takahashi, Kazutaka Ikeda, Makoto Arita

Research output: Contribution to journalArticle

Abstract

Illuminating the comprehensive lipid profiles after dietary supplementation of polyunsaturated fatty acids (PUFAs) is crucial to revealing the tissue distribution of PUFAs in living organisms, as well as to providing novel insights into lipid metabolism. Here, we performed lipidomic analyses on mouse plasma and nine tissues, including the liver, kidney, brain, white adipose, heart, lung, small intestine, skeletal muscle, and spleen, with the dietary intake conditions of arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) as the ethyl ester form. We incorporated targeted and untargeted approaches for profiling oxylipins and complex lipids such as glycerol (phospho) lipids, sphingolipids, and sterols, respectively, which led to the characterization of 1026 lipid molecules from the mouse tissues. The lipidomic analysis indicated that the intake of PUFAs strongly impacted the lipid profiles of metabolic organs such as the liver and kidney, while causing less impact on the brain. Moreover, we revealed a unique lipid modulation in most tissues, where phospholipids containing linoleic acid were significantly decreased in mice on the ARA-supplemented diet, and bis(monoacylglycero)phosphate (BMP) selectively incorporated DHA over ARA and EPA. We comprehensively studied the lipid profiles after dietary intake of PUFAs, which gives insight into lipid metabolism and nutrition research on PUFA supplementation.

Original languageEnglish
Article number241
JournalMetabolites
Volume9
Issue number10
DOIs
Publication statusPublished - 2019 Oct

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Unsaturated Fatty Acids
Lipids
Arachidonic Acid
Tissue
Eicosapentaenoic Acid
Docosahexaenoic Acids
Nutrition
Lipid Metabolism
Liver
Brain
Oxylipins
Kidney
Sphingolipids
Metabolome
Sterols
Linoleic Acid
Tissue Distribution
Dietary Supplements
Glycerol
Small Intestine

Keywords

  • Arachidonic acid
  • Dietary fat
  • Fatty acid metabolism
  • Lipidomics
  • Mass spectrometry
  • Omega-3 fatty acids

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

Cite this

Characterization of lipid profiles after dietary intake of polyunsaturated fatty acids using integrated untargeted and targeted lipidomics. / Naoe, Satoko; Tsugawa, Hiroshi; Takahashi, Mikiko; Ikeda, Kazutaka; Arita, Makoto.

In: Metabolites, Vol. 9, No. 10, 241, 10.2019.

Research output: Contribution to journalArticle

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