Characterization of monocyte-macrophage-lineage cells induced from CD34+ bone marrow cells in vitro

Kyoko Suzuki, Nobutaka Kiyokawa, Tomoko Taguchi, Hisami Takenouchi, Masahiro Saito, Toshiaki Shimizu, Hajime Okita, Junichiro Fujimoto

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5 Citations (Scopus)

Abstract

We characterized the expression of cell surface antigens and cytokine-secreting ability of monocyte-macrophage-lineage cells induced in vitro from CD34+ bone marrow cells. After cultivation for 3 weeks, we observed 2 distinct cell fractions: a floating small, round cell fraction and an adherent large, protruding cell fraction. Both cell fractions expressed myelocyte-monocyte-lineage antigens, but mature-macrophage markers such as CD206 were expressed only by the adherent cells. An assessment of cells cultured for 5 weeks revealed spontaneous secretion of interleukin 8 (IL-8) and IL-6, and lipopolysaccharide (LPS)-induced tumor necrosis factor α (TNF-α) secretion in both fractions, but only the adherent cell fraction secreted IL-10 after LPS stimulation. In contrast, both fractions of cells cultured for 3 weeks spontaneously secreted low levels of IL-8, but none of the other cytokines. Upon LPS stimulation, the cells secreted IL-6 and TNF-α, but not IL-10. We also assessed the effect of granulocyte colony-stimulating factor (G-CSF) pretreatment on TNF-α secretion by each cell fraction and found that G-CSF reduced TNF-α secretion only in the adherent fraction of cells cultured for 3 weeks. Monocyte-macrophage-lineage cells induced in vitro should provide an ideal model for functional analysis of monocyte-macrophage cells.

Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalInternational journal of hematology
Volume85
Issue number5
DOIs
Publication statusPublished - 2007 Jun 1
Externally publishedYes

Keywords

  • Antigen
  • Cytokine
  • Expression
  • Monocyte-macrophage lineage

ASJC Scopus subject areas

  • Hematology

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    Suzuki, K., Kiyokawa, N., Taguchi, T., Takenouchi, H., Saito, M., Shimizu, T., Okita, H., & Fujimoto, J. (2007). Characterization of monocyte-macrophage-lineage cells induced from CD34+ bone marrow cells in vitro. International journal of hematology, 85(5), 384-389. https://doi.org/10.1532/IJH97.06213