Abstract
We characterized the expression of cell surface antigens and cytokine-secreting ability of monocyte-macrophage-lineage cells induced in vitro from CD34+ bone marrow cells. After cultivation for 3 weeks, we observed 2 distinct cell fractions: a floating small, round cell fraction and an adherent large, protruding cell fraction. Both cell fractions expressed myelocyte-monocyte-lineage antigens, but mature-macrophage markers such as CD206 were expressed only by the adherent cells. An assessment of cells cultured for 5 weeks revealed spontaneous secretion of interleukin 8 (IL-8) and IL-6, and lipopolysaccharide (LPS)-induced tumor necrosis factor α (TNF-α) secretion in both fractions, but only the adherent cell fraction secreted IL-10 after LPS stimulation. In contrast, both fractions of cells cultured for 3 weeks spontaneously secreted low levels of IL-8, but none of the other cytokines. Upon LPS stimulation, the cells secreted IL-6 and TNF-α, but not IL-10. We also assessed the effect of granulocyte colony-stimulating factor (G-CSF) pretreatment on TNF-α secretion by each cell fraction and found that G-CSF reduced TNF-α secretion only in the adherent fraction of cells cultured for 3 weeks. Monocyte-macrophage-lineage cells induced in vitro should provide an ideal model for functional analysis of monocyte-macrophage cells.
| Original language | English |
|---|---|
| Pages (from-to) | 384-389 |
| Number of pages | 6 |
| Journal | International Journal of Hematology |
| Volume | 85 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2007 Jun |
| Externally published | Yes |
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Keywords
- Antigen
- Cytokine
- Expression
- Monocyte-macrophage lineage
ASJC Scopus subject areas
- Hematology
Cite this
Characterization of monocyte-macrophage-lineage cells induced from CD34+ bone marrow cells in vitro. / Suzuki, Kyoko; Kiyokawa, Nobutaka; Taguchi, Tomoko; Takenouchi, Hisami; Saito, Masahiro; Shimizu, Toshiaki; Okita, Hajime; Fujimoto, Junichiro.
In: International Journal of Hematology, Vol. 85, No. 5, 06.2007, p. 384-389.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of monocyte-macrophage-lineage cells induced from CD34+ bone marrow cells in vitro
AU - Suzuki, Kyoko
AU - Kiyokawa, Nobutaka
AU - Taguchi, Tomoko
AU - Takenouchi, Hisami
AU - Saito, Masahiro
AU - Shimizu, Toshiaki
AU - Okita, Hajime
AU - Fujimoto, Junichiro
PY - 2007/6
Y1 - 2007/6
N2 - We characterized the expression of cell surface antigens and cytokine-secreting ability of monocyte-macrophage-lineage cells induced in vitro from CD34+ bone marrow cells. After cultivation for 3 weeks, we observed 2 distinct cell fractions: a floating small, round cell fraction and an adherent large, protruding cell fraction. Both cell fractions expressed myelocyte-monocyte-lineage antigens, but mature-macrophage markers such as CD206 were expressed only by the adherent cells. An assessment of cells cultured for 5 weeks revealed spontaneous secretion of interleukin 8 (IL-8) and IL-6, and lipopolysaccharide (LPS)-induced tumor necrosis factor α (TNF-α) secretion in both fractions, but only the adherent cell fraction secreted IL-10 after LPS stimulation. In contrast, both fractions of cells cultured for 3 weeks spontaneously secreted low levels of IL-8, but none of the other cytokines. Upon LPS stimulation, the cells secreted IL-6 and TNF-α, but not IL-10. We also assessed the effect of granulocyte colony-stimulating factor (G-CSF) pretreatment on TNF-α secretion by each cell fraction and found that G-CSF reduced TNF-α secretion only in the adherent fraction of cells cultured for 3 weeks. Monocyte-macrophage-lineage cells induced in vitro should provide an ideal model for functional analysis of monocyte-macrophage cells.
AB - We characterized the expression of cell surface antigens and cytokine-secreting ability of monocyte-macrophage-lineage cells induced in vitro from CD34+ bone marrow cells. After cultivation for 3 weeks, we observed 2 distinct cell fractions: a floating small, round cell fraction and an adherent large, protruding cell fraction. Both cell fractions expressed myelocyte-monocyte-lineage antigens, but mature-macrophage markers such as CD206 were expressed only by the adherent cells. An assessment of cells cultured for 5 weeks revealed spontaneous secretion of interleukin 8 (IL-8) and IL-6, and lipopolysaccharide (LPS)-induced tumor necrosis factor α (TNF-α) secretion in both fractions, but only the adherent cell fraction secreted IL-10 after LPS stimulation. In contrast, both fractions of cells cultured for 3 weeks spontaneously secreted low levels of IL-8, but none of the other cytokines. Upon LPS stimulation, the cells secreted IL-6 and TNF-α, but not IL-10. We also assessed the effect of granulocyte colony-stimulating factor (G-CSF) pretreatment on TNF-α secretion by each cell fraction and found that G-CSF reduced TNF-α secretion only in the adherent fraction of cells cultured for 3 weeks. Monocyte-macrophage-lineage cells induced in vitro should provide an ideal model for functional analysis of monocyte-macrophage cells.
KW - Antigen
KW - Cytokine
KW - Expression
KW - Monocyte-macrophage lineage
UR - http://www.scopus.com/inward/record.url?scp=34548380903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548380903&partnerID=8YFLogxK
U2 - 10.1532/IJH97.06213
DO - 10.1532/IJH97.06213
M3 - Article
VL - 85
SP - 384
EP - 389
JO - International Journal of Hematology
JF - International Journal of Hematology
SN - 0925-5710
IS - 5
ER -